Objective:To investigate the effect of Propofol on expression of microRNA-15b(miR-15b)and vascular endothelial cells apoptosis in hypoxia/reoxygenation cell model.Methods:The hypoxia/reoxygenation cell model[human umbilical vein endothelial cells(HUVECs)]was established.The experimental group,the control group and the blank group were set up.HUVECs were incubated with hypoxia/reoxygenation for 24h,then treated with 150μmol/L Propofol for 6h.The expression of miR-15bin each group of HUVECs was detected by real-time quantitative PCR(RT-qPCR).The expression of B lymphoblastoma-2 (bcl-2)expression were detected by RTqPCR and Western-blot.Flow cytometry was used to detect apoptosis in each group.Results:RT-qPCR results showed that the expression level of miR-15bin the experimental group was lower than that in the control group(P<0.05).The results of RT-qPCR and Western-blot showed that the expression of bcl-2mRNA and protein in the experimental group were higher than that in the control(P<0.05).The apoptosis rate of HUVECs in experimental group was lower than that in control group(P<0.05).Conclusion:Propofol can inhibit the expression of miR-15b in hypoxia/reoxygenation HUVECs and affect the expression of its target gene bcl-2(anti-apoptotic protein,thus reducing the apoptosis rate.%目的:研究丙泊酚在缺氧/复氧细胞模型中对微小RNA-15b(miR-15b)及血管内皮细胞凋亡的影响.方法:建立缺氧/复氧细胞模型[人脐静脉内皮细胞(HUVECs)],设置实验组、对照组、空白组,其中实验组HUVECs缺氧/复氧培养24h后,加入丙泊酚(150μmol/L)干预培养6h;实时定量PCR(RT-qPCR)检测各组HUVECs中miR-15b的表达情况;RT-qPCR、Western-blot检测各组HUVECs中miR-15b靶基因B淋巴细胞瘤-2(bcl-2)的表达情况;流式细胞术检测各组细胞凋亡能力.结果:RT-qPCR结果显示,实验组miR-15b的表达水平低于对照组(P<0.05);RT-qPCR及Western-blot结果显示,实验组bcl-2在mRNA和蛋白水平的表达均高于对照组(P<0.05);实验组HUVECs的细胞凋亡率低于对照组(P<0.05).结论:丙泊酚能够抑制缺氧/复氧HUVECs细胞中miR-15b的表达,并影响其靶基因抗凋亡蛋白bcl-2的表达,从而降低细胞凋亡率.
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