采用组织分离的方法从野生侧耳属菌株（Pleurotu ssp．）子实体上分离得到菌株 XZG －ts，以其基因组为模板扩增获得该菌株的 ITS 序列，序列分析结果表明，菌株 XZG －ts 与 GenBank 中来自于澳大利亚、印度和中国云南的3个肺形侧耳菌株聚为一簇，组内核苷酸一致率为100％，而与侧耳属其他4个种的10个菌株核苷酸一致率为98．10％～99．06％，依此结果将 XZG －ts 菌株鉴定为 P．pulmonarius。进一步研究了不同碳源、氮源、碳氮组合、温度及 pH 值对菌株 XZG －ts 菌丝生长的影响，结果表明，最适碳源为果糖，氮源为酵母膏，最佳碳氮源组合为果糖1％、麦芽糖1．0％、酵母膏0．2％、蛋白胨0．3％，最适生长温度为25℃，最适 pH值为6。%A fungi strain,XZG -ts,collected from the Mountain Tai,was isolated by tissue isolation and the internal transcribed spacers (ITS)was amplified by PCR.In the phylogenetic tree constructed with ITS,XZG -ts formed a branch with 3 isolates belonging to Pleurotus pulmonarius from Australian,India and China with the consistent rate of 100% in intra -group nucleotide.While it was only 98.10% ~99.06% with other 10 isolates of Pleurotus.Therefore XZG -ts was identified as P.pulmonarius.The effects of carbon source,nitrogen source,carbon -nitrogen combination and pH value on the growth of mycelium were further studied.The results showed the optimum carbon source,nitrogen source,temperature and pH value was fruc-tose,yeast extract,25℃ and 6,respectively.And the optimum carbon -nitrogen combination was 1% fruc-tose,1.0% maltose,0.2% yeast extract,0.3% peptone.
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