The donor parents, transgenic insect - resistant Indica rice TT51 with cry1Ab/1Ac,T2A - 1 with cry2A and T1C - 19 with cry1C from Minghui 63 and Japonica rice RJ -5 with cry1C from Zhonghua 11 were transferred into the main acceptor parents Shengdao 13 ,Shengdao 15 and Zhendao 88 cultivated in HuangHuai area by successive backcrossing. Three lines from TT51 with cry1Ab/1Ac, two lines from T2A - 1 with cry2A,three lines from T1C -19 with crylC and two lines from RJ - 5 with cry1C were selected based on the Basta detection, PCR identification and field insect - resistance selection. These lines performed better insect -resistance and high -yielding characters in field, so it laid foundation for breeding transgenic rice varieties with insect resistance in HuangHuai area.%利用黄淮稻区主栽品种圣稻13、圣稻15、镇稻88为受体亲本,选择以籼稻明恢63为背景的转基因抗虫材料TT51(cry1Ab/1Ac)、T2A-1(cry2A)、T1C-19(cry1C)和以粳稻中花11为背景的转基因抗虫材料J-5(cry1C)为供体,分别进行杂交和多代回交.每一世代后代单株,通过涂抹Basta抗性筛选,结合PCR鉴定跟踪抗虫目的基因以及田间抗虫性鉴定、农艺性状选择,培育出来源于TT51带有cry1Ab/1Ac基因的抗虫稳定株系3个,来源于T2A-1带有cry2A基因的稳定株系2个,来源于T1C-19带有cry1C基因的稳定株系3个,来源于J-5带有cry1C基因的抗虫稳定株系2个.这些株系于田间均表现出很好的抗虫性状和优质丰产性状,为黄淮稻区抗虫转基因水稻育种奠定了基础.
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