A HPLC method was developed for the quantitative determitation of IPP. The mobile phase was a 20:30:50mixture of acetonitrile、methanol and water,and the flow rate was 1 mL/min. A C18 chromatographic column was used and the detection wave length was 354 nm. The regression equation was y=44. 834x-306. 49 ,the correlationg coefficient of the standard curve was 0. 9999. The average recovery was 99. 83%,the standard deviation was 0. 013,and the variation coefficient was 0. 0133%. The method was simple ,rapid and had high precision and good linear correlation.%本文建立了一种高效液相色谱法测定哌虫啶的定量分析方法。该方法采用Agilent ZORBAX SB-C18色谱柱，以V（乙腈）：V（甲醇）：V（水）=20：30：50三元混配体系作为流动相，流速为1mL/min，在波长354 nm下，对哌虫啶进行定量分析。结果表明：该分析方法的线性回归方程为y=44.834x-306.49，相关系数r为0.9999，平均回收率为99.83％，标准偏差为0.013，变异系数为0.0133％。该方法简便快捷，准确度高，线性关系良好。
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