目的 探讨铜绿假单胞菌(PA)注射液对肺癌细胞增殖、凋亡的影响,并探讨其机制.方法 选择肺癌细胞株NCI-H1395和95-D接种于96孔板,分别设对照组和观察1、2、3组,分别加入0、0.25×109/mL、0.5×109/mL、1×109/mL的PA注射液培养24 h.另设对照组、PA组和AG1478组,对照组不加药,PA组加入1×109/mL的PA,AG1478组加入1×109/mL的PA和10-7 mol/L的表皮生长因子受体抑制剂AG1478.采用MTT法观察各组细胞增殖情况;AnnexinV/PI双染试验检测各组细胞凋亡率.结果 随着PA浓度的升高,肺癌NCI-H1395和95-D细胞OD 570不断降低,细胞凋亡率不断升高(P均<0.05).与对照组相比,PA组细胞OD 570降低(P<0.05),细胞凋亡率升高(P<0.05);与PA组相比,AG1478组细胞OD 570升高,细胞凋亡率降低(P均<0.05).结论 PA注射液通过EGFR信号通路抑制肺癌细胞NCI-H1395和95-D增殖,促进其凋亡.%Objective To explore the effects of pseudomonas aeruginosa (PA)injection on the cell proliferation and apoptosis of lung cancer cells,and to explore its mechanism. Methods NCI-H1395 and 95-D of lung cancer cell lines were selected and then were inoculated in 96-well plate. We divided them into the control group and observation groups 1, 2,and 3,which were separately added with 0,0. 25 × 109 ,0. 5 × 109 ,and 1 × 109 / mL PA injection for 24 h. In addi-tion,the control group,PA group,and AG1478 group were set. The drug was not added into the control group,1 × 109 PA was added into the PA group,and 1 × 109 / mL PA and 10 - 7 mol/ L EGFR inhibitor AG1478 were added into the AG1478 group. The proliferation of cells was observed by MTT. The apoptosis rate of each group was detected by the AnnexinV/ PI double dye test. Results With the increase of PA concentration,the OD 570 of NCI-H1395 and 95-D cells was decreasing, and the cell apoptosis rate increased (all P < 0. 05). Compared with the control group,the OD 570 of PA group decreased (P < 0. 05),while the apoptosis rate increased (P < 0. 05). Compared with the PA group,the OD 570 of AG1478 in-creased,and the apoptosis rate decreased (both P < 0. 05). Conclusion PA injection can inhibit the proliferation of NCI-H1395 and 95-D of lung cancer cells and promote apoptosis through EGFR signaling pathway.
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