目的：观察人抗原R（ HuR）和缺氧诱导因子（ HIF）-1α在肝癌组织中的表达变化，并探讨其相关性。方法取48例肝癌组织及癌旁组织标本，采用免疫组化染色法检测肝癌组织及癌旁组织中HuR和HIF-1α蛋白的表达，采用荧光定量PCR法检测肝癌组织中HIF-1αmRNA的表达。结果肝癌组织中HuR和HIF-1α蛋白阳性表达率分别为67％和58％，显著高于癌旁组织的19％和10％（ P均＜0．01）。肝癌组织中HuR与HIF-1α蛋白的表达呈正相关（r＝0．723，P＜0．01）。 HuR蛋白高表达者中HIF-1αmRNA的表达较HuR蛋白低表达者显著上调（P＜0．05）。结论肝癌组织中HuR和HIF-1α蛋白表达上调且呈正相关关系，HuR可能增强HIF-1αmRNA的稳定性，从而促进其表达。%Objective To investigate the expression and correlation of human antigen R ( HuR) and hypoxia-induc-ible factor (HIF)-1αin hepatocellular carcinoma (HCC) tissues.Methods Forty-eight cases of HCC tissue and adjacent tissue samples were collected from patients undergoing surgical resection .The expression of HuR and HIF-1αprotein in the HCC and adjacent tissues was detected by immunohistochemical staining .The mRNA expression of HIF-1αin the HCC tis-sues was detected by fluorescent quantitative PCR .Results The positive expression rates of HuR and HIF-1αprotein in the HCC tissues were 67%and 58%, respectively .These rates were significantly higher than the positive expression rates of HuR and HIF-1αprotein (19%and 10 %) in the adjacent tissues ( all P<0.01) .The expression of HuR protein was positively correlated with the expression of HIF-1αprotein in the HCC tissues (r=0.723, P<0.01).The mRNA expres-sion of HIF-1αmRNA in the group with high expression of HuR protein was significantly up-regulated as compared with that of the group with low expression of HuR protein (P<0.05).Conclusions The expression of HuR and HIF-1αprotein was up-regulated and positively correlated with each other in the HCC tissues .HuR may enhance the stability of HIF-1αmRNA, and thus increase the expression of HIF-1αprotein.
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