目的：观察贲门腺癌（GCA）组织中Raf激酶抑制蛋白（RKIP）和核因子-κBp65（NF-κBp65）的表达变化，并探讨其意义。方法160例GCA患者，手术时留取癌组织（腺癌组）及癌旁组织（癌旁组），采用免疫组化法检测标本中RKIP、NF-κBp65，并分析两指标间及与GCA临床病理特征的关系。结果腺癌组及癌旁组RKIP阳性表达率分别为38．75％、75．00％，两组比较，P＜0．01；NF-κBp65阳性表达率分别为75．625％、20．000％，两组比较，P＜0．01。 RKIP阳性表达与GCA淋巴结转移有关（P＜0．05）；NF-κBp65阳性表达与GCA临床分期、肿瘤分化程度、淋巴结转移有关（P均＜0．05）。 GCA组织中RKIP与NF-κBp65表达呈负相关（r＝-0．431，P＜0．01）。结论 GCA组织中RKIP表达下调、NF-κBp65表达上升，RKIP表达减少或缺失可能通过上调NF-κBp65的表达促进GCA的侵袭和转移。%Objective To observe the expression changes of Raf kinase inhibitor protein ( RKIP) and nuclear factor-κBp65 (NF-κBp65) in gastric cardia adenocarcinoma (GCA) and to discuss the significance.Methods The expression of RKIP and NF-κBp65 in cancerous tissues ( carcinoma group ) and the paracancerous tissues ( adjacent group ) from 160 GCA patients were detected by immunohistochemistry assay .The relationships between the two indexes and with the clinical and pathological features of GCA were analyzed .Results The positive expression rates of RKIP in the cancerous tissues and paracancerous tissues were 38.75%and 52.5%, respectively;the difference was significant (P<0.01).The positive expression rates of NF-κBp65 in the two groups were 75 .625% and 20 .000%, and the difference was significant ( P<0.01).The positive expression rate of RKIP was associated with lymph node metastasis in GCA (P<0.05).The positive expression rate of NF-κBp65 was associated with clinical staging , differentiation of the cancer and lymph node metastasis (all P<0.05).Negative correlation was found between the expression of RKIP and NF-κBp65 in GCA (r=-0.431, P<0.01).Conclusions The expression of RKIP is down-regulated and NF-κBp65 is up-regulated in the GCA tissues .The invasion and metastasis of GCA are promoted by the up-regulation of NF-p65 expression and down-regulation RKIP expres-sion.
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