目的 应用透明质酸(HA)制备缓释载药微球包被软骨源性形态发生蛋白-l(CDMP-1)后,行兔膝关节腔内注射,观察其对兔膝骨关节炎的治疗作用.方法 在实验兔双后膝关节腔内注射木瓜蛋白酶溶液造模.造模后第3、6周,分别向各实验兔关节腔内注射1 mL生理盐水(模型组)、1 mL CDMP-1溶液(CDMP-1溶液组)、1 mL含PBS的微球(HA微球组)、1 mL含CDMP-1的微球(HA/CDMP-1微球组),于第9周处死动物后取材.以未处理动物作为空白对照组.对各组软骨进行大体形态及组织病理学评价；采用RT-PCR方法对软骨组织中MMP-13mRNA、TIMP-1 mRNA表达量进行检测.结果 模型组关节软骨损伤程度较对照组明显加重；与模型组相比,CDMP-1溶液组和HA微球组关节软骨损伤程度无明显减轻；HA/CDMP-1微球组关节软骨损伤程度较模型组明显减轻.结论 关节腔内注射HA/CDMP-1微球可促进关节软骨损伤修复,抑制骨关节炎的进展.%Objective Cartilage-derived morphogenetic protein-1 (CDMP-1) was prepared by applying hyaluronic acid (HA) , and then it was injected into the rabbit knee joint cavity, to observe the therapeutic effects on inhibiting osteoarthritis progress. Methods The model was made by injecting papain in the double knee joint cavities. After modeling for the first 3 and 6 weeks, all experimental groups were injected with 1 mL of rabbit articular saline (model group) , 1 mL CDMP-1 solution (CDMP-1-S group) , 1 mL PBS containing microspheres (HA-M group) and 1 mL microspheres with CDMP-1 (HA/CDMP-1-M group). At week 9, rats were killed. The untreated rats were used as blank control group. The general form and histopathology of the cartilage were evaluated; MMP-13, TIMP-1 mRNA expression changes were detected with RT-PCR. Results Compared with the control group, articular cartilage damage in model group was significantly increased; there was no significant articular cartilage damage mitigation in CDMP-1-S group and the HA-M group as compared with the model group. But, articular cartilage damage in HA/CDMP-1-M group as compared with the model group was significantly reduced. Conclusion Intra-articular injection with HA/CDMP-1-M can promote cartilage repairation and inhibit the progress of osteoarthritis.
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