Objective To investigate the effect of dexamethasone (Dex) on proliferation and apoptosis in glioma C6 cells. Methods The glioma C6 cells were divided into 4 groups. The cell number in each group was 1. 25×106. Group B、C、D were cultured with Dex, except group A. The final concentraion of Dex in B、C、D group was 10 ,10-4,10-3 mol/L. The number of C6 cells were counted by ordinary counting chamber, the cell cycle and the apoptotic rate of C6 cells were detected by flow cytometry. Results After cocultured for 24 h, the number of C6 glioma cells in group A,B,C,D were 4. 37 × 106, 4. 29 × 106, 3. 57 × 106 and 3.44 × 106, there were remarkable differences between group B, C, D and group A( all P < 0. 05 ) . The apoptosis rate in these 4 groups were 0. 37% , 0. 52% , 0. 39% and 8. 24% , there were remarkable differences between group A, B, C, D and group D(all P<0.05). The proportion of C6 cells in the phase Go/ G1 were 82.42% , 93.21% , 93.71% , 77.52% , in the phase S were 13.22% , 5.38% , 4.06% , 14.74% ,in the phase G2/M were 4. 36% , 1.41% , 2. 23% , 7. 74%. There were remarkable differences between group B, C and group A in the phase Go/G, , S(all P <0. 05). There was remarkable difference between A, B, C group and D group in the phase G2/M (all P<0.05). Conclusion Dex can inhibite cell proliferation and induce apoptosis by inhibiting C6 cell cycle progression.%目的 观察地塞米松(Dex)对胶质瘤C6细胞增殖和凋亡的影响.方法 将C6细胞分为A、B、C、D组,每组1.25×106个细胞,A组不加Dex,B、C、D组分别与终浓度为10-5、10-4、10-3 mol/L的Dex共培养；用血计数板计算C6细胞数,用流式细胞仪检测C6细胞周期及凋亡率.结果 培养24 h时,A、B、C、D组C6细胞数依次为4.37×106、4.29×106、3.57×106、3.44 ×106个/瓶,B、C、D组与A组比较,P均＜0.05;C6细胞凋亡率依次为0.37％、0.52％、1.39％、8.24％,D组与A、B、C组比较,P均＜0.05.G0/G1期C6细胞所占比例分别为82.42％、93.21％、93.71％、77.52％,S期分别为13.22％、5.38％、4.06％、14.74％,G2/M期分别为4.36％、1.41％、2.23％、7.74％；B、C组与A组G0/G1、S期细胞比例比较,P均＜0.05;D组与A、B、C组G2/M期细胞比例比较,P均＜0.05.结论 Dex可通过阻滞C6细胞周期进程抑制细胞增殖、诱导细胞凋亡.
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