Objective To observe the effect of hypoxia-inducible factor 2(HIF-2)small interference RNA(siRNA) on the proliferation and apoptosis in human pancreatic carcinoma BXPC-3 cells. Methods BXPC-3 cells were divided into group A, B and C randomly. Cells in group A were transfected with HIF-2 siRNA, in group B with nonspecific siRNA and group C uninfected. Cells in each group were cultured with normoxic environment and hypoxic state. The cell proliferation was assessed by MTT assay at 24, 48, 72, 96 h and the changes in cell apoptosis were evaluated by flow cytometry at 48 h. Results At the 48 h of hypoxic state, the OD value of group A, B and C were respectively 0. 54 ± 0. 07 , 0. 69 ± 0. 09, 0. 72 ± 0.13 , at the 72 h are respectively 0. 55 ± 0. 11, 0. 88 ± 0. 07, 0. 88 ± 0. 05 , at 96 h were respectively 0. 56 ± 0.12, 0. 93 ± 0. 11, 0. 94 ± 0. 09. Compared with group B and C, cell proliferation in group A was inhibited remarkably (all P < 0. 05 ) . At the 48 h of hypoxic state culture, the apoptosis rate of group A, B and C were respectively 21. 37% ± 0. 17% , 7. 12% ±0. 03% , 7. 24% ±0. 07% . Compared with group B and C, the apoptosis in group A was increased significantly ( all P < 0. 05 ) . In normoxic environment, there was no significant difference between the OD value and apoptosis rates of each group. Contusion In hypoxic environment, HIF-2 siRNA can inhibit cell proliferation, promote apoptosis in human pancreatic carcinoma cells.%目的 观察靶向缺氧诱导因子2 (HIF-2)的小干扰RNA (siRNA)对人胰腺癌BXPC-3细胞增殖和凋亡的影响.方法 将BXPC-3细胞随机分为A、B、C组,A组转染HIF-2 siRNA、B组转染非特异性siRNA、C组不转染,分别予常氧、缺氧环境下培养；采用MTT法检测培养24、48、72、96 h时BXPC-3细胞的光密度(OD)值观察增殖情况,用流式细胞仪检测培养48 h时BXPC-3细胞凋亡率.结果 在缺氧培养48 h时,A、B、C组细胞OD值分别为0.54 ±0.07、0.69 ±0.09、0.72 ±0.13,72 h时分别为0.55±0.11、0.88 ±0.07、0.88±0.05,96 h时分别为0.56±0.12、0.93 ±0.11、0.94 ±0.09；A组与B、C组比较,P均＜0.05.在缺氧培养48 h时,A、B、C组细胞凋亡率分别为21.37％±0.17％、7.12％±0.03％、7.24％±0.07％；A组与B、C组比较,P均＜0.05.在常氧状态下,各组细胞OD值及凋亡率无明显差别.结论 缺氧状态下,靶向HIF-2 siRNA可抑制人胰腺癌BXPC-3细胞增殖,诱导其凋亡.
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