Objective To investigate the expression and significance of miR-214 in adrenal cortical tumor tissues. Methods Real-time quantitative polymerase chain reaction (PCR) was used to detect the levels of miR-214 expression in adrenal cortical tumor tissues, adjacent non-tumorous tissues, human adrenal cortical carcinoma SW-13 cell lines (SW-13 cell lines) and nude mice transplanted tumor tissues. Results The levels of miR-214 expression in adrenal cortical carcinoma tissues, SW-13 cell lines, nude mice transplanted tumor tissues were significant lower compared with adrenal cortical adenoma tissues and adjacent non-tumorous tissues (all P <0.01); the levels of miR-214 expression in nude mice transplanted tumor tissues were lower than those in adrenal cortical carcinoma tissues and SW-13 cell lines (all P <0.05). Conclusions Down-regulation of miR-214 expression may be involved in the occurrence and development of adrenal cortical carcinoma, and further exploring its function and mechanism has important significance.%目的 探讨miR-214在肾上腺皮质肿瘤组织中的表达及意义.方法 选择21例行肾上腺皮质肿瘤手术患者的术后切除组织,其中肾上腺皮质腺瘤组织8份,其瘤旁正常组织8份,肾上腺皮质癌组织5份；另选人肾上腺皮质癌SW-13细胞系(SW-13细胞系)和裸鼠皮下移植瘤组织6份.采用RT-PCR技术检测各组织中的miR-214表达.结果 与肾上腺皮质腺瘤组织和瘤旁正常组织比较,肾上腺皮质癌组织、SW-13细胞系、裸鼠皮下移植瘤组织中的MiR-214表达明显降低(P均＜0.01)；与肾上腺皮质癌组织和SW-13细胞系比较,裸鼠皮下移植瘤组织中的MiR-214表达降低(P＜0.05).结论 miR-214表达下调可能参与肾上腺皮质癌的发生、发展,深入探讨其功能和作用机制有重要意义.
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