目的 观察卵泡抑素(FS)、激活素(Activin)A与骨形态发生蛋白(BMP)-4在大鼠脑发育过程中的表达变化规律.方法 将同期受孕30只SD大鼠按照胎鼠发育时间随机分为胚胎8.5 d(E8.5组)、13 d(E13组)、18d(E18组)及出生后3 d(P3组)、7d(P7组)、30 d(P30组)各5只,采用免疫组化ABC法检测各组脑皮质、纹状体、海马齿状回、嗅球组织中FS、Activin A、BMP-4表达情况.结果 FS与Activin A在大鼠脑内广泛分布,二者在E8.5组表达最高,并以E13组表达强度开始降低,在P30组降至最低,同一发育阶段各脑区表达无明显差异；在大鼠的相应脑区BMP-4亦广泛表达,但从E8.5组到P7组持续低表达,尤以海马表达极弱,P30组在不同脑区呈高表达,各发育阶段以大脑皮质和纹状体表达略强.结论 FS、Activin A与BMP-4在大鼠不同发育年龄各脑区呈波动性表达,表达水平与发育年龄密切相关.%Objective To observe the variation rule of follistatin ( FS) , Activin A and bone morphogenetic protein (BMP)-4 expression during the brain development of rat. Methods Thirty simultaneous pregnant SD rats were randomly divided into six groups, with five in each group; embryo 8. 5 d( E8. 5 group) , embryo 13 d( E13 group) , embryo 18 d (E18 group) and 3 ds after born(P3 group) , 7 ds after born(P7 group) , 30 ds after bom(P30 group). Expressions of FS, Activin A and BMP4- in the cortex, striatum, hippocampus and olfactory bulb were examined by means of ABC immu-nohistochemical method. Results FS and Activin A expression level in rat brain was strong in E8.5 group, the level began to descend at E13 group, and decreased to the minimum at P30 group. In the same phase, no distinct differences patterns were found in different tissues. BMP-4 was widely expressed in rat brain, but from E 8. 5 group to P7 group appeared with a lower level, especially weak in the hippocampus. The positive cells in P30 group were abundant at different brain regions, and which in the cortex and striatum were stronger in the same phase. Conclusion The expression of follistatin, Activin A and BMP-4 in different brain regions changes at different ages, and which is closely correlated with the developmental age.
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