Objective To explore the relationship between vitamin D and acute pulmonary edema in mice, and to investigate its effects in the process of acute pulmonary edema.Methods Acute lung edema mouse model was established by i.p.injection LPS.BALB/c male mice were treated with active vitamin D analogue paricalcitol (every other day i.p.injection)for 2 weeks.To analyze the survival time,wet-dry ratio,lung index,the level of TNF-α,IL-6,the activity of MPO and histological examination in lung tissues.Results Vitamin D treated lung e-dema mice showed higher survival time than the vehicle-treated controls(P <0.05).The pathological injury was reduced by vitamin D.The wet-dry ratio and lung index was lower in vitamin D-treated mice compared with con-trol group.The levels of TNF-α,IL-6 and the activity of MPO were significantly down-regulated by vitamin D (P<0.01 or P <0.05).Conclusion These observations suggest that vitamin D could improve the pathological chan-ges in acute pulmonary edema mice,which may be related to the reduced release of inflammatory factors.The clin-ical application of vitamin D therapy may ameliorate pulmonary edema of patients..%目的：探讨维生素 D 与急性肺水肿小鼠之间的关系，以明确其在急性肺水肿中的作用及机制。方法用脂多糖腹腔注射方法制作急性肺水肿小鼠模型，给予雄性 BALB／c 小鼠活性维生素 D 类似物帕立骨化醇腹腔注射2周，设立空白及模型对照组，分析各组小鼠生存时间，观察小鼠肺组织湿质量／干质量比，肺系数，肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6水平，髓过氧化物酶（MPO）的活性，并做肺组织病理切片。结果维生素 D 处理组肺水肿小鼠相对于对照组生存时间延长（P ＜0．05）；病理损伤改善，湿质量／干质量比，肺系数有所降低；TNF-α、IL-6水平，MPO 的活性均降低（P ＜0．01或 P ＜0．05）。结论维生素 D 能够缓解急性肺水肿小鼠的病理改变，其机制可能与减少炎症因子释放有关。临床上应用维生素 D 治疗可能会减轻患者的肺水肿。
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