Objective To establish methods for bioactivity detection of recombinant human serum albumin-interferon α2b fusion protein for injection .Methods Bioactivity was assayed by WISH-VSV system ,taking meth-ods for bioactivity detection of recombinant human interferon as reference .Results The methods were specific . The RSD value in the precision test was 10.6% ,and the average recovery rate was 98.2% with RSD 9.0% .Con-clusion The established methods were specific and accurate ,which can be used to determine the bioactivity of re-combinant human serum albumin-interferonα2b fusion protein for injection .%目的：通过对注射用重组人血清白蛋白-干扰素α2b融合蛋白的生物学活性检测方法进行研究，建立该药物的生物学活性检测方法。方法参考重组人干扰素生物学活性测定法，以人羊膜细胞（WISH ）病变抑制法开展注射用重组人血清白蛋白-干扰素α2b融合蛋白的生物学活性检测方法研究。结果该方法的专属性好，精密度试验中生物学活性相对标准偏差（RSD）为10.6％，准确性试验平均回收率98.2％，RSD为9.0％，符合生物制品质量检验方法要求。结论本研究制定的实验方法准确、可靠，适用于注射用重组人血清白蛋白-干扰素α2b融合蛋白的生物学活性检测。
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