为了解重庆市某牛场牛群大肠杆菌O157∶H7的感染情况，我们以牛场犊牛为研究对象，采集其新鲜粪便，经LB培养基增菌培养、免疫磁珠富集后，在山梨醇麦康凯琼脂上划线培养，最后PCR扩增eaeA基因目的片段，以此分离鉴定粪源大肠杆菌O157∶H7。结果显示：在所采集的39份犊牛粪便样品中，分离得到一株符合大肠杆菌O157∶H7生长特点且含有eaeA基因的菌株。%In order to understand the E.coli O157∶H7 infectious status of a cattle herd in Chongqing,fresh droppings of calves were collected to incubate on LB medium. The E.coli O157∶H7 on sorbitol MacConkey agar streak cultured after seperating by immunomagnetic sand method. Finally, eaeA gene fragment was amplified by PCR to ensure the isolation and identification of E.coli O157∶H7 in accuracy. The result showed that there was one strain which consistent with growth character-istics of E.coli O157∶H7 and containing eaeA gene in all 39 faeces samples.
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