An analytical method based on high performance liquid chromatography (HPLC)has been established for the simultaneous determination of 3 benzalkonium chloridehomologs in mucosa disinfectants.The HPLC separation was carried out on a CN(250 mm×4.6mm,5μm)column,the mobile phase were acetonitrile-0.1％ammonium acetate buffer solution(45 ∶ 55,v/v),the flow rate was 1.0mL/min,the detection wavelength was 214 nm,the column temperature was 25 ℃C,and the injection volume was 20μL.The limit of detection was 1301μg/g and the quantitation limit was 435μg/g for benzalkonium chloride.The linear range ofbenzalkonium chloride was 5μg/mL-200μg/mL.The method is simple,rapid,accurate and suitable for the determination of 3 benzalkonium chloride homologs in Oral ulcer paste.%建立了高效液相色谱法同时检测口腔溃疡膏中3种苯扎氯铵同系物的方法.色谱柱:CN柱(250mm×4.6mm,5μm);流动相:乙腈-0.1 mol/L醋酸铵缓冲溶液(45∶55,v/v);流速:1.0mL/min;检测波长:214nm;柱温:25℃;进样量:20μL.该方法对苯扎氯铵的检出浓度为130μg/g,最低定量浓度为435μg/g,苯扎氯铵在5μg/mL～200μg/mL浓度范围内线性关系良好.该方法快速、准确,能够同时测定口腔溃疡膏中3种苯扎氯铵同系物.
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