This experiment was established for the simultaneous determination of five kinds of free anthraquinone methods of Qiming tablets.Chromatographic column is DiamonsiL C18 (4.6mm×250mm,5μm),acetonitrile-0.1％ phosphoric acid solution as mobile phase,gradient elution (0min-20min,40 ∶ 60;20min-30min,60;40;30min-50min,90 ∶ 10),the detection wavelength of 284nm 1.0mL/min,flow rate,column temperature of 25℃.The linear ranges were in concentration of 0.001 36 mg/mL-0.00 680mg/mL for ehrysophanol,in concentration of 0.000 65mg/mL-0.003 25mg/mL for emodin,in concentration of 0.000 13mg/mL-0.000 65mg/mL for physcion,in concentration of 0.000 96mg/mL-0.004 80mg/mL for obtusifolin,in concentration of 0.000 54 mg/mL-0.002 70mg/mL for aurantio-ob-tusin,in precision and stability;repeat the test,RSD ＜ 1％;the average recovery rate was 99.73％,99.53％,99.31％,99.69％,99.33％.the method is simple and feasible,of good reproducibility and high accuracy,which can be used as a method for simultaneous determination of five kinds of anthraquinone in the Qiming tablets.%本实验建立了同时测定杞明片中5种蒽醌的方法.色谱柱:DiamonsiLC18 (4.6mm×250mm,5μm);流动相:乙腈-0.1％磷酸溶液,梯度洗脱(0min～20min,40∶60;20min～30min,60∶40;30min～50min,90:10);检测波长:284nm;流速:1.0mL/min;柱温:25℃.橙黄决明素在0.001 36mg/mL～0.006 80mg/mL、大黄酸在0.000 65mg/mL～0.003 25mg/mL、大黄素在0.000 13mg/mL～0.000 65mg/mL、大黄酚在0.000 96mg/mL～0.004 80mg/mL、大黄素甲醚在0.000 54mgnL～0.002 70mg/mL范围内呈良好的线性关系;精密度、稳定性、重复性试验的RSD＜1％;平均回收率分别为99.73％、99.53％、99.31％、99.69％、99.33％.所用方法简便可行、重现性好、准确度高,可作为同时测定杞明片中5种蒽醌含量的方法.
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