This study was attempt to determine rapid propagation methods for Rheum lhasaense A.J.Li et P.K.Hsiao,then establish theregeneration system for R.lhasaense A.J.Li et P.K.Hsiao through callus induction.R.lhasaense A.J.Li et P.K.Hsiao seed as material,Tissue culture method was used to induce the callus ofR.lhasaense.Results show:peeled seeds is the best explant for tissue culture;High concentration of cytokinin and relatively low amount of 2,4-D were beneficial to the induction and differentiation of shoot;MS+6-BA1.0mg/L+KT1.0mg/L+ZT0.5 mg/L+2,4-D0.5mg/Lis the best medium of induction and differentiation of primary culture;MS+6-BA2.0mg/L+KT2.0mg/L+ZT1.0mg/L+2,4-D0.5mg/Lis the best medium for proliferation;1/2 MS+ NAA1.0mg/L + 2000mg/Lacticarbon is the best rooting inducing medium,and the rooting rate was 98％.The highest survival rate was 90％.%建立拉萨大黄快速繁殖体系,并通过诱导愈伤组织建立拉萨大黄再生体系.以拉萨大黄种子为材料,利用组织培养方法对拉萨大黄进行愈伤组织诱导.结果表明,剥皮种子是拉萨大黄组织培养的最佳外植体;较高浓度的细胞分裂素和相对较低的2,4-D有利于丛生芽的诱导及分化;MS+6-BA 1.0mg/L+KT 1.0mg/L+ZT0.5mg/L+2,4-D0.5mg/L是最佳的初代培养诱导及分化培养基;MS+6-BA2.0mg/L+KT2.0mg/L+ZT 1.0mg/L+2,4-D0.5mg/L为最佳的增殖培养基;1/2MS+NAA 1.0mg/L+2000mg/L活性炭是最佳的生根诱导培养基,生根率98％;炼苗成活率达到90％.
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