采用高效液相色谱－质谱联用法（HPLC－ESI－MSn ）对不同品种山葡萄中5种花色苷成分进行快速定量分析研究，比较5个山葡萄品种样品中花色苷的含量差异。色谱柱：PFP Kinetix （2.1mm ×50mm ，2.6μm ）；流动相：2％甲酸水溶液－2％甲酸乙腈溶液梯度洗脱；质谱负离子模式检测；选用多离子反应通道监控模式（MRM ）。结果显示，该方法准确、快速、重现性好、分辨率高，可很好地用于山葡萄及相关产品中花色苷单糖苷的定量分析。%The aim of the present study is to analyze five anthocyanins in Vitis amurensis Rupr .by HPLC-ESI-MSn .And the contents of five anthocyanins in five different V .amurensis Rupr .species were compared .Column:PFP Kinetix (2.1mm × 50mm ,2.6μm );mobile phase:2%formic acid water and 2% formic acid acetonitrile under gradient elution .The ESI-MS was carried out in negative ion mode and multiple reac-tion monitoring (MRM ) .The results demonstrated that the method developed was accurate and rapid with good reproducibility and high resolu-tion .This method could be applied in the quantitative analysis of anthocyanins in V .amurensis Rupr .and related products .
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