To develop a method for the content determination of astragnaloside in Chai Shi Jie Yu tablets.The chromatography column was oc-tadecylsilane chemically bonded silica as the filler ,the moving phase was acetonitrile -water(32:68) ,the flow velocity was 1mL one minute , the drift tube temperature of evaporative light -scattering detector was 105℃ ,air or nitrogen was as the atomizing gas ,its flow velocity was 3.0L one minute.Results:The incoming sample amount of astragnaloside was 1.024μg-5.12μg ,the average recovery was 97.59% ,RSD=0.92% (n=6 ).This method is sensitive ,accurate ,and has good reproducibility.It can be used for the content determination of astragnaloside in Chai Shi Jie Yu tablet.%建立以HPLC-ELSD法测定柴石解郁片中黄芪甲苷含量的方法.色谱柱 :Diamonsil C18 ;流动相:乙腈-水(32:68 );流速 :1mL/min;蒸发光散色器漂移管温度 :105℃ ;雾化气体 :氮气 ;流速 :3.0L/min.结果表明 ,黄芪甲苷线性范围1.024μg~5.12μg ,平均回收率97.59% ,RSD=0.92% (n=6 ).本方法灵敏、准确、重现性好 ,可用于柴石解郁片的质量控制.
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