为进一步提高梅花鹿人工输精受胎率，本试验对梅花鹿发情持续时间进行了观测，并进行了二次输精试验。结果表明，梅花鹿发情持续时间平均为18.35h ±2.97h ，范围是14h～25.53h ，发情持续时间14h≤a＜22h时段占90.32％，22h≤a＜26h时段占9.68％；在梅花鹿发情持续期末进行1次冷冻解冻精液输精的受胎率为64.3％；在梅花鹿首次发情后12h进行第1次输精，第1次输精后4.5h～5h进行第2次输精，受胎率81.6％。2次输精较1次输精受胎率提高17.3％。根据梅花鹿发情持续时间分布规律，采取2次输精方法是提高梅花鹿受胎率的可行方法。%To further improving conception rate of artificial insemination in sika deer ,this study investigated estrus duration and two times of re-peat insemination method in sika deer .The results showed that estrus duration was 18.35h ± 2.97h ,rang of variation was 14h-25.53h ,it was 90.32% in the during of 14h≤a<22h ,it was 9.68% in the during of 22h≤a<26h ,the estrous duration of sika deer mainly concentrated in the during of 14h≤a<22h;Pregnancy rate was 81.6% using two times of repeat insemination method ,it was 64.3% using once insemination method ,and it was increased by 17.3% comparing once insemination .This study has confirmed that if twice insemination method is to be adopted ,it will be a feasible way to improve pregnancy rate according to the estrus duration distribution of sika deer .
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