We designed three experiments according to higher blood levels of deer antler tissue and more severe ossification ,2D-clean up puri-fied protein was best extraction method for two -dimensional electrophoresis of sika deer antlers .% 根据鹿茸组织血液含量较高、骨化较为严重的特点，设计了3种适合双向电泳的鹿茸蛋白质提取方法。筛选出2D －cleanup纯化为鹿茸蛋白质双向电泳的最佳提取方法。
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