Fluorescence spectroscopy was used to investigate the influences of carbon nanotubes (CNTs) on the fluorescence of bovine serum albumin (BSA),the influences of CNTs on that of gatifloxacin (GFLX) , and the influences of GFLX on that of BSA with or without coexisting CNTs under imitated physiological condition. The experimental results demonstrate that both gatifloxacin and carbon nanotubes could quench the intrinsic fluorescence of BSA, and the quenching mechanism is mainly static quenching. The fluorescence quenching action of GFLX on BSA was weakened in the presence of CNTs. The fluorescence quenching data were analyzed according to Stern-Volmer equation and double-reciprocal Lineweaver-Burk equation. It was shown that the quenching constant (Ksv) and the binding constant (K) are decreased with the concentration of carbon nanotubes increasing. The effects of coexisting CNTs on GFLX-BSA interactions were discussed to offer a reference for the studies on the action mechanism of CNTs or GFLX with albumins in vivo.%在模拟生理条件下,用荧光光谱法研究了碳纳米管对牛血清白蛋白和加替沙星荧光光谱特性的影响以及有无碳纳米管共存时加替沙星对牛血清白蛋白荧光光谱特性的影响.实验结果表明,加替沙星和碳纳米管都可以使牛血清白蛋白的荧光强度发生静态猝灭.在碳纳米管的存在下,加替沙星与牛血清白蛋白的结合作用有所减弱.Stern-Volmer荧光猝灭图和Lineweaver-Burk双倒数图计算的结果表明,加替沙星和牛血清白蛋白之间的猝灭常数Ksv和结合常数K均随碳纳米管浓度的增大而减小.通过对三者结合作用的研究,为探讨碳纳米管、加替沙星在生物体内与蛋白质相互作用的机理提供了理论依据.
展开▼
