Objective In order to determine the factors pertaining to the development and metabolism of periodontal ligament, the author investigated the effects of human osteoblast cells ( Obs ) on human periodontal ligament fibrohlast cells ( HPLFs). Methods The co-culture system of Obs and human HPLFs separated by transwell was established. The proliferation and alkaline phosphatase( AL Pase) activity of HPLF were evaluated by cell number counter and enzyme kinetics methods after 1,3,5,7 days. Results After co-culture,the number of HPLFs were 3. 5 x 10 3 'days later and 7. 5 x 10 5 days later. The difference between the two groups was significant (P < 0.05). The ALP activities of HPLFs increased by co-culture with human Obs 3,5,7 days later. Conclusions Human Obs could restrain the proliferation of HPLFs,while induce the differentiation of HPLFs.%目的 观察成骨细胞( osteoblast cells,OBs)对人牙周膜成纤维细胞(human periodontal ligament fibroblast cells,HPLFs)生物学特性的影响,为进一步探讨正畸牙齿移动的生物学机制奠定基础.方法建立人OBs与HPLFs共培养系统,通过细胞计数及生化检测法观察成骨细胞对人牙周膜成纤维细胞增殖和分化的影响.结果 3d和5d时,共培养组HPLFs细胞计数分别为3.5×104个/ml及7.5×104个/ml,均明显低于对照组HPLFs细胞计数,且差异有统计学意义(P＜0.05).HPLFs碱性磷酸酶(ALP)活性比较中,transwell共培养组HPLFs ALP活性高于对照组.在3d时,差异有统计学意义(P＜0.05),5d及7d时差异也有统计学意义(P＜0.叭).结论 人OBs可能抑制HPLFs的增殖,促进HPLFs ALP活性.
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