OBJECTIVE Development of a novel electrochemical biosensor for the determination of glu-cose.METHODS A novel electrochemical platform for the high sensitivity detection of glucose was constructed by gold nanoparticles-polydopamine-thionine-graphene oxide ( Au – PDA – THi – GO )/chitosan/glucose oxidase ( GOD) nanocomposite film modified glassy carbon electrode.RESULTS The present electrochemical biosensor ex-hibited a wide linear range from 0.02mM~2.42mM for detection of glucose,with a detection limit of 6.7μm(S/N=3).CONCLUSION This method is simple,economic,green,and the prepared biosensor showed high sensitivity long-term stability and good reproducibility.%目的 研制一种新型葡萄糖电化学生物传感器. 方法 通过原位化学聚合法制备了多功能纳米金-聚多巴胺-硫堇-石墨烯( Au-PDA-THi-GO)功能复合纳米材料,并结合壳聚糖( CS)固定葡萄糖氧化酶( GOD)构建电化学葡萄糖生物传感器. 结果 该传感器对葡萄糖检测的响应电流与其浓度在0.02mM~2.42mM范围内成良好的线性关系,线性相关系数为0.996,检出限为6.7μm(S/N=3). 结论 该方法简单、经济、绿色,并且制备的葡萄糖生物传感器具有灵敏性高、稳定性好和使用寿命长等特点.
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