目的 研究蛋白磷酸酯酶2A(protein phosphatase 2A,PP2A)抑制剂冈田酸(okadaic acid,OA)对胎鼠海马神经元轴突生长的影响,探讨PP2A在神经元轴突生成中的作用.方法 实验分为二甲基亚砜(Dimethyl sulfoxide,DMSO)对照组和OA处理组,OA处理组依药物浓度又分为5、10、20、30、40 nmol/L 5个组,激光扫描共聚焦显微镜和普通光学显微镜观察海马神经元形态变化.结果 对照组原代海马神经元培养48 h后神经元轴突已经形成.当给予不同浓度的OA处理后普通光学显微镜下可见原代海马神经元突起生长均明显受到抑制,突起长度缩短随OA浓度增加抑制作用逐渐增强,对照组平均轴突长度为185 μm,而不同浓度OA处理后则分别下降至150 μm、100 μm、80 μm、75 μm、50 μm.结论 OA抑制原代海马神经元轴突生长,提示PP2A在神经元轴突生成中起重要作用.%Objective To explore the possible role of protein phosphatase 2A in the outgrowth of hipp-ocampal neuron by Okadaic acid (OA) , a widely used phosphatase inhibitor that is thought to selectively inhibit protein phosphatase 2A(PP2A). Methods Primary hippocampal neurons were treated with DMSO as control and OA with five concentration, 5、10、20、30、40 nM, In OA treated groups, the hippocampal neurons were administered with OA from 24 h to 48 h after culturing for 24 h. The morphological changes of the neurons were observed through light microscope and laser scanning confocal microscope. Results The neurons formed axons after culturing for 48h, however, the axons outgrowth were inhibited significantly when treated with OA in dose dependent manner. Statistical analysis result showed that the normal average axon length was 185μm, but the average length decreased to 150 μrn、100 μm、80 μm、75 μm、50 μm, respectively. Conclusions The result that okadaic acid inhibits the formation of axons, which implicates PP2A may play an important role in neuronal polarity.
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