Molecular mapping of a novel wheat powdery mildew resistance gene Ml92145E8-9 and its application in wheat breeding by marker-assisted selection

Xiaocheng Yu; Shizhong Ren; Lanfei Zhao; Jun Guo; Yinguang Bao; Yingxue Ma; Hongwei Wang; Herbert W.Ohm; Dazhao Yu; Hongjie Li; Lingrang Kong

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Molecular mapping of a novel wheat powdery mildew resistance gene Ml92145E8-9 and its application in wheat breeding by marker-assisted selection

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Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is one of the most devastating diseases of common wheat(Triticum aestivum L.).The wheat line 92145E8-9 is immune to Bgt isolate E09.Genetic analysis reveals that the powdery mildew resistance in 92145E8-9 is controlled by a single dominant gene,temporarily designated Ml92145E8-9.Bulkedsegregant analysis(BSA)with simple sequence repeat(SSR)markers indicates that Ml92145E8-9 is located on chromosome 2AL.According to the reactions of 92145E8-9,VPM1(Pm4b carrier),and Lankao 906(PmLK906 carrier)to 14 Bgt isolates,the resistance spectrum of 92145E8-9 differs from those of Pm4b and PmLK906,both of which were previously localized to 2AL.To test the allelism among Ml92145E8-9,Pm4b and PmLK906,two F2 populations of 92145E8-9×VPM1(Pm4b)and 92145E8-9×Lankao 906(PmLK906)were developed in this study.Screening of 784 F2 progeny of 92145E8-9×VPM1 and 973 F2 progeny of 92145E8-9×Lankao 906 for Bgt isolate E09 identified 37 and 19 susceptible plants,respectively.These findings indicated that Ml92145E8-9 is non-allelic to either Pm4b or PmLK906.Thus,Ml92145E8-9 is likely to be a new powdery mildew resistance gene on 2AL.New polymorphic markers were developed based on the collinearity of genomic regions of Ml92145E8-9 with the reference sequences of the International Wheat Genome Sequencing Consortium(IWGSC).Ml92145E8-9 was mapped to a 3.6 cM interval flanked by molecular markers Xsdauk13 and Xsdauk682.This study also developed five powdery mildew-resistant wheat lines(SDAU3561,SDAU3562,SDAU4173,SDAU4174,and SDAU4175)using flanking marker-aided selection.The markers closely linked to Ml92145E8-9 would be useful in marker-assisted selection for wheat powdery mildew resistance breeding.

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《作物学报：英文版》 |2018年第6期|P.621-627|共7页
作者
Xiaocheng Yu; Shizhong Ren; Lanfei Zhao; Jun Guo; Yinguang Bao; Yingxue Ma; Hongwei Wang; Herbert W.Ohm; Dazhao Yu; Hongjie Li; Lingrang Kong;
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作者单位

[1]State Key Laboratory of Crop Biology;

College of Agronomy;

Shandong Agricultural University;

Tai''''an 271018;

Shandong;

China;

[1]State Key Laboratory of Crop Biology;

College of Agronomy;

Shandong Agricultural University;

Tai''''an 271018;

Shandong;

China;

[1]State Key Laboratory of Crop Biology;

College of Agronomy;

Shandong Agricultural University;

Tai''''an 271018;

Shandong;

China;

[1]State Key Laboratory of Crop Biology;

College of Agronomy;

Shandong Agricultural University;

Tai''''an 271018;

Shandong;

China;

[1]State Key Laboratory of Crop Biology;

College of Agronomy;

Shandong Agricultural University;

Tai''''an 271018;

Shandong;

China;

[1]State Key Laboratory of Crop Biology;

College of Agronomy;

Shandong Agricultural University;

Tai''''an 271018;

Shandong;

China;

[1]State Key Laboratory of Crop Biology;

College of Agronomy;

Shandong Agricultural University;

Tai''''an 271018;

Shandong;

China;

[2]Department of Agronomy;

Purdue University;

West Lafayette;

IN 47907-1150;

USA;

[3]Hubei Academy of Agricultural Sciences;

Wuhan 430064;

Hubei;

China;

[4]Institute of Crop Sciences;

Chinese Academy of Agricultural Sciences;

Beijing 100081;

China;

[1]State Key Laboratory of Crop Biology;

College of Agronomy;

Shandong Agricultural University;

Tai''''an 271018;

Shandong;

China;

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原文格式 PDF
正文语种 CHI
中图分类农业科学;
关键词
Marker-assisted selection; Ml92145E8-9; Powdery mildew; Triticum aestivum L.;

机译：标记辅助选择;Ml92145E8-9;白粉病;普通小麦;

Molecular mapping of a novel wheat powdery mildew resistance gene Ml92145E8-9 and its application in wheat breeding by marker-assisted selection

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