Polymerase chain reaction and denaturing gel gradient electrophoresis method (hereafter abbreviated as PCR-DGGE) is getting a larger range of application. China has a slow start in this field but it is still gradually taken seriously and researched and applied by people. This paper analyzes the application and prospect of this in the field of environmental engineering.%聚合酶链式反映和变性梯度凝胶电泳相结合的方法（以下英文简称：PCR-DGGE）逐渐应用于各个领域，使用范围也越来越广。我国在该领域起步较慢，但是，也逐渐被大家重视、研究并加以利用。本文就该技术在环境工程领域中的应用和前景进行分析。
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