目的 应用Ⅲ类磁力矫治器建立青春期恒河猴动物模型,观察其对颅面部形态学及颅面骨缝的组织学影响,并探讨其机制.方法 选用生长发育期雄性恒河猴6只,实验组45、90d各2只,对照组45、90d各1只.实验组配戴铸造式磁力矫治器,对照组不戴用矫治器.采用头颅侧位片测量分析其颅颌面的形态学变化.选用颅面部6条骨缝作为观察对象,应用活体荧光染色及苏木精-伊红染色观察其组织学变化.结果 所有实验动物颌位均出现明显近远中移位,90d组的变化较45 d组更为明显,对照组无明显变化.荧光染色及苏木精-伊红染色观察显示:实验组各骨缝均表现为明显的骨沉积,尤其是颧颌缝、腭横缝、翼腭缝、颧颞缝最为明显.结论 Ⅲ类磁力矫治器可以引起上下颌骨以及牙齿位置的变化,并可使颅面骨缝内纤维结缔组织排列方向发生变化,导致骨缝两侧新骨的大量沉积.%Objective To investigate the influence of class ID forces in craniofacial morphology and sutural histology in Rhesus monkeys. Methods Six male Rhesus monkeys in mixed dentition were employed in this study, two as 90-day experimental group, two as 45-day experimental group and the other two as control respectively. The experimental monkeys were casted magnetic orthopedic appliance-El (MOA-DI), the control monkeys received no treatment. Intraoral photographs, casts and cephalometric radiographs of each animal were obtained at the beginning and at the end of the experiment. Fluorescent and haematoxylin-eosin staining were used to observe the histological changes in craniofacial sutures. Results In experimental groups, the maxillomandibular relationship changed more significantly in 90-day experimental group than 45-day experimental animals. But changes were scarcely noted in the control. The bony depositions of the sutures were distinctly found during the experimental period, especially in zygomaticomaxillary, transverse palatine, pterygopalatine and zygomaticotemporal sutures. Conclusion This study indicates that this kind of magnetic appliance could cause skeletal and dental changes of maxilla and mandible, and change the arrangement of connective tissues in craniofacial sutures, and lead to significant new bone deposition in craniofacial sutures.
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