目的：通过观察针刺捻转补泻手法对自发性高血压大鼠（Spontaneously Hypertensive Rats，SHR）血浆及肾组织中肾素、血管紧张素Ⅱ（AngiotensinⅡ，AngⅡ）的影响，探讨捻转补泻手法对自发性高血压大鼠肾脏保护的可能机制。方法：雄性 SHR 大鼠60只随机分为模型对照组（A）、针刺留针组（B）、捻转补法组（C）、捻转泻法组（D）和平补平泻组（E），每组12只。12只 WKY 大鼠作为空白组（F）。各针刺组分别采用不同的手法针刺“太冲穴”，模型对照组和空白组只做与针刺组相同抓捉刺激，不做针刺处理。治疗结束后，取全血和大鼠肾脏，采用 ELISA 法检测各组大鼠血浆及肾组织中肾素、AngⅡ含量，光镜下观察肾组织病理学变化。结果：模型对照组血浆及肾组织肾素、AngⅡ含量升高，与空白组比较差异有统计学意义（P ＜0.01），与捻转补法组、平补平泻组和针刺留针组比较差异有统计学意义（P ＜0.05）。捻转泻法组血浆及肾组织肾素、AngⅡ含量明显降低，与捻转补法组、平补平泻组、针刺留针组和模型对照组差异有统计学意义（P ＜0.01），与空白组比较差异无统计学意义（P ＞0.05）。结论：捻转泻法能有效降低 SHR 大鼠血浆及肾组织肾素、血浆 AngⅡ含量。捻转泻法对 SHR 大鼠肾脏的保护作用可能与循环及组织中肾素、AngⅡ含量的变化有关。%Objective:To observe how twirling reinforcing and reducing method protect the kidney in spontaneously hypertensive rats (SHR)by recording its influence on the levels of renin and Angiotensin (AngII).Methods:Sixty males SHR were randomly divided into five groups:model control group (group A),acupuncture group (group B),acupuncture with reinforcing method group (group C),acupuncture with reducing method group (group D)and even reinforcing-reducing method group (group E). Twelve WKY rats were taken as blank group (group F).Each group adopted different acupuncture manipulations on LR3 (Tai Chong).The model and blank groups were just given acupuncture-like stimulus.After the treatment,the whole blood and renal tissues were taken from all rats,then to detect levels of renin and Ang II by ELISA and observe the pathological changes of renal tissues in light microscope.Results:Compared with the blank group,the levels of renin and AngII in blood plasma and kidney of SHR in model group were higher(P <0.01 ).Also,compared with reinforcing method group,reinforcing-reducing method group and acupuncture group,the results were of statistical difference(P <0.05).The levels of renin and AngII of SHR in reducing method group were significantly declined,and when compared with reinforcing method group,reinforcing-reducing method group, acupuncture group and model group,there existed statistical difference(P <0.01 ).No significant differences exist between reduc-ing method group and blank group(P >0.05).Conclusion:Twirling reduction method of acupuncture can effectively reduce the levels of renin and AngⅡin blood plasma and kidney in SHR,which would explain its mechanism of kidney protection.
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