目的：探讨HTR3B基因多态性与妇科腹腔镜患者术后恶心呕吐（PONV）发生率的相关性。方法选择255例妇科腹腔镜手术患者，根据是否发生恶心呕吐分为呕吐组和非呕吐组。采用DNA直接测序HTR3B基因中rs1185027，rs4936285，rs3758987，rs12795805四个单核苷酸多态性位点在两组患者中的等位基因和基因型分布，结果呕吐组rs3758987位点TT基因型分布频率较无呕吐组低，呕吐组T等位基因频率显著低于无呕吐组（P<0.05，OR=1.60，95%CI=1.00~2.55）。呕吐组连锁不平衡分析发现单倍型（rs1185027- rs3758987- rs4936285），随访期间rs3758987不同基因型术后呕吐发生率存在差异，log-rank检验统计量为19.34，P<0.05。Kaplan-Meier分析结果显示，不同基因型之间呕吐累积发生率有显著差异（log-rank=19.34，P<0.05）。结论 HTR3B基因rs3758987位点多态性与中国汉族女性人群PONV发生率具有相关性。%ObjectiveThis study investigated whether common genomic variations of the HTR3B are associated with the incidence of postoperative vomiting in patients who underwent gynecological laparoscopic surgery.MethodsWe conducted a prospective observational study design of 255 consecutive patients who underwent laparoscopic surgery. According to whether there will be vomiting, divided into vomit group and non-vomit group. All of the patients were direct DNA sequencing was performed to detect polymorphisms of HTR3B(rs1185027,rs4936285,rs3758987, rs12795805).ResultsFour single nucleotide polymorphisms(SNPs)in HTR3B were found.One of these(rs3758987 in HTR3B)was statistically associatedwith vomiting,The TT genetype of vomit group were lower than non-vomit group,The T allele in the frequency of the two group were different,the difference was statistically signifi cant.(P<0.05,OR=1.60,95%CI=1.00~2.55). In the analysis of Kaplan-Meier,The vomiting rates of genotypes exhibited signifi cantly different(log-rank=19.34,P<0.05).Conclusions The HTR3B rs3758987 SNP might serve as a predictorof postoperative vomiting in patients who undergoing gynecological laparoscopic surgery.
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