目的 探讨解偶联蛋白2(UCP2) 45bp-I/D基因多态性与代谢综合征(MetS)的关系.方法 选取杭州市采荷小区40岁以上汉族人群作为研究对象,符合2005年国际糖尿病联盟(IDF)MetS诊断标准者纳入MetS-IDF组,共95例;符合2013年中华医学会糖尿病学分会(CDS)MetS诊断标准者纳入MetS-CDS组,共78例;代谢指标完全正常者为正常组(N组),共109例,并进行UCP2基因多态性分型,分析不同诊断标准MetS组与N组间基因型的区别,并行Binary logistic回归分析不同基因型中MetS各相关组分的区别.结果 UCP2基因频率在MetS-IDF组为D/D 72.63％、D/I+I/1 27.37％,MetS-CDS组为D/D 78.21％、D/I+I/I21.79％,N组为D/D 88.99％、D/I+I/I 11.01％;等位基因I频率在MetS-IDF组为16.32％、MetS-CDS组为13.46％、N组为7.8％.不同诊断标准的两组MetS患者基因型分布和等位基因频率与N组对比差异均有统计学意义(均P＜0.05).Binary logistic回归分析表明,MetS-IDF组中,基因型为D/I+I/I者相对于D/D者发生高甘油三酯血症、胰岛素抵抗、腰围增粗等风险增高,MetS-CDS组中,基因型为D/I+I/I者相对于D/D者发生高甘油三酯血症、胰岛素抵抗、腰围增粗、BMI升高风险增加.结论 两个MetS诊断标准均显示,MetS患者与代谢指标正常者的UCP2 45bp-I/D基因型分布和等位基因频率均有显著差异,I等位基因可能是MetS的危险因素.%Objective To investigate the relationship between polymorphisms of uncoupling protein 2 (UCP2) gene and metabolic syndrome (MetS) in Han Chinese population.Methods Total 760 Han Chinese residents from one community in Hangzhou were randomly selected in the study.Demographic data were collected,and metabolic index were detected.Among 760 individuals,95 met the MetS criteria of 2005 International Diabetes Federation (IDF)(MetS-IDF group) and 78 met the MetS criteria of 2013 Chinese Diabetes Society (CDS)(MetS-CDS group),109 subjects with normal blood tests and physical examination served as control group.The genotypes of the UCP2 45bp-I/D were determined with PCR-RFLP.The influencing factors of MetS were analyzed by logistic Binary regression.Results The gene frequency of D/D in MetS-IDF group and control group was 72.63％ and 88.99％(P＜0.05),that of D/I+I/I was 27.37％ and 11.01％(P＜0.05),respectively;the frequency of allele I in MetS group and control group was 16.32％ and 7.8％(P＜0.05)respectively.The gene frequency of D/D in MetS-CDS group and control group was 78.21％ and 88.99％(P＜0.05),respectively,that of D/I+I/I was 21.79％ and 11.01％(P＜0.05),respectively,the frequency of allele I in MetS group and control group was 13.46％ and 7.8％(P＜0.05),respectively.Binary logistic analysis showed that the subjects with D/I+I/I genotype had higher risks of hypertriglyceridemia,insulin resistance and increased waist circumference than those with D/D genotype in MetS-IDF group.While the subjects with D/I+I/I genotype had higher risks of the hypertriglyceridemia,insulin resistance,increased waist circumference and body mass index(BMI)than those with D/D genotype in MetS-CDS group.Conclusion Our study shows that the allele I of UCP2 gene might be a risk factor for metabolic syndrome in Han Chinese population.
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