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In Crystallo Capture of a CovalentIntermediate in the UDP-Galactopyranose Mutase Reaction

机译：在共价的结晶捕获中UDP-半乳糖苷酶突变的中间产物

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UDP-galactopyranose mutase (UGM) plays an essential role in galactofuranose biosynthesis in pathogens by catalyzing the conversion of UDP-galactopyranose to UDP-galactofuranose. Here we report the first crystal structure of a covalent intermediate in the UGM reaction. The 2.3 Å resolution structure reveals UDP bound in the active site and galactopyranose linked to the FAD through a covalent bond between the anomeric C of galactopyranose and N5 of the FAD. The structure confirms the role of the flavin as nucleophile and supports the hypothesis that the proton destined for O5 of galactofuranose is shuttled from N5 of the FAD via O4 of the FAD.

机译：UDP-吡喃半乳糖突变酶（UGM）通过催化UDP-吡喃半乳糖转化为UDP-吡喃半乳糖而在病原体中半呋喃呋喃糖生物合成中发挥重要作用。在这里，我们报告了UGM反应中共价中间体的第一个晶体结构。 2.3Å分辨率结构揭示了UDP结合在活性位点中，并且通过吡喃半乳糖的异头C与FAD的N5之间的共价键连接到FAD的吡喃半乳糖。该结构证实了黄素作为亲核试剂的作用，并支持了假说，即以半乳糖呋喃糖为O5的质子通过FAD的O4从FAD的N5穿梭。

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期刊名称 ACS AuthorChoice
作者
Ritcha Mehra-Chaudhary; Yumin Dai; Pablo Sobrado; *; John J. Tanner; *;
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年(卷),期 -1(55),6
年度 -1
页码 833–836
总页数 4
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1. In Crystallo Capture of a Covalent Intermediate in the UDP-Galactopyranose Mutase Reaction [J] . Mehra-Chaudhary Ritcha, Dai Yumin, Sobrado Pablo, Biochemistry . 2016,第6期

机译：在共价中间体的UDP-Galactopyranose突变酶反应的结晶捕获中。
2. Crystal Structures of Mycobacteria tuberculosis and Klebsiella pneumoniae UDP-Galactopyranose Mutase in the Oxidised State and Klebsiella pneumoniae UDP-Galactopyranose Mutase in the (Active) Reduced State. [J] . Beis K, Srikannathasan V, Liu H, Journal of Molecular Biology . 2005,第4期

机译：处于氧化态的肺结核分枝杆菌和肺炎克雷伯菌UDP-半乳糖吡喃糖突变酶的晶体结构和处于（活性）还原态的肺炎克雷伯菌UDP-半乳糖吡喃糖酶的晶体结构。
3. Multiple functionalities of reduced flavin in the non-redox reaction catalyzed by UDP-galactopyranose mutase [J] . Sobrado Pablo, Tanner John J. Archives of Biochemistry and Biophysics . 2017,第期

机译：用UDP-半乳糖烷烃催化催化的非氧化还原反应中的黄芪的多种函数
4. Toward Accurate Modelling of Enzymatic Reactions: All Electron Quantum Chemical Analysis combined with QM/MM Calculation of Chorismate Mutase [C] . Toyokazu Ishida International Conference of Computational Methods in Sciences and Engineering . 2008

机译：旨在精确建模的酶反应：所有电子量子化学分析与QM / MM计算合并核酸异位异质酶
5. CRISPR/Cas9-induced Genetic Disruption and Characterization of UDP-Galactopyranose Mutase in Trypanosoma cruzi [D] . Manriquez Roman, Claudia 2018

机译：CRISPR / Cas9诱导的克鲁氏锥虫UDP-半乳糖吡喃糖基因突变的遗传特征
6. QM/MM Molecular Dynamics Study of the Galactopyranose → Galactofuranose Reaction Catalysed by Trypanosoma cruzi UDP-Galactopyranose Mutase [O] . Gustavo Pierdominici-Sottile, Rodrigo Cossio Pérez, Johan F. Galindo, 2010

机译：克鲁氏锥虫UDP-半乳糖苷酶催化半乳糖吡喃糖→半乳糖呋喃糖的QM / MM分子动力学研究
7. In Crystallo Capture of a Covalent Intermediate in the UDP-Galactopyranose Mutase Reaction [O] . Ritcha Mehra-Chaudhary, Yumin Dai, Pablo Sobrado, 2016

机译：在UDP-半乳糖烷烃蛋黄反应中的共价中间体的晶体捕获中
8. Neutron Capture Studies: 1, Multiple Capture Reactions and Implications for Calculated beta-Delayed Fission Rates: 2, The Nuclear Level Structure of 238Np [R] . Hoff, R. W. 1988

机译：中子俘获研究：1，多次捕获反应及其对计算β延迟裂变率的影响：2，238Np的核级结构

In Crystallo Capture of a CovalentIntermediate in the UDP-Galactopyranose Mutase Reaction

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