Crystallization and preliminary X-ray crystallographic studies of a Lys49-phospholipase A2 homologue from Bothrops pirajai venom complexed with rosmarinic acid

机译：结合迷迭香酸的Bothrops pirajai毒液中Lys49-磷脂酶A2同源物的结晶和初步X射线晶体学研究

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摘要

PrTX-I, a noncatalytic and myotoxic Lys49-phospholipase A2 from Bothrops pirajai venom, was crystallized in the presence of the inhibitor rosmarinic acid (RA). This is the active compound in the methanolic extract of Cordia verbenacea, a plant that is largely used in Brazilian folk medicine. The crystals diffracted X-rays to 1.8 Å resolution and the structure was solved by molecular-replacement techniques, showing electron density that corresponds to RA molecules at the entrance to the hydrophobic channel. The crystals belong to space group P212121, indicating conformational changes in the structure after ligand binding: the crystals of all apo Lys49-phospholipase A2 structures belong to space group P3121, while the crystals of complexed structures belong to space groups P21 or P212121.

机译：在抑制剂迷迭香酸（RA）存在下，PrTX-1（一种来自Bothrops pirajai毒液的无催化毒性和肌毒性的Lys49-磷脂酶A2）被结晶。这是马鞭草（Cordia verbenacea）甲醇提取物中的活性化合物，该植物主要用于巴西民间医学中。晶体将X射线衍射至1.8Å分辨率，并通过分子置换技术解析了结构，显示出与疏水通道入口处的RA分子相对应的电子密度。晶体属于空间群P212121，表明配体结合后结构的构象变化：所有apo Lys49-磷脂酶A2结构的晶体都属于空间群P3121，而复杂结构的晶体属于空间群P21或P212121。

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期刊名称 Acta Crystallographica Section F: Structural Biology and Crystallization Communications
作者
Juliana I. dos Santos; Norival A. Santos-Filho; Andreimar M. Soares; Marcos R. M. Fontes;
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作者单位

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年(卷),期 2010(66),Pt 6
年度 2010
页码 699–701
总页数 3
原文格式 PDF
正文语种
中图分类分子生物学;生化遗传学;生化药理学;生物物理学;
关键词
phospholipase A2 Bothrops pirajai venom myotoxins rosmarinic acid Cordia verbenacea;

机译：磷脂酶A2;Bothrops pirajai毒液;肌毒素;迷迭香酸;马鞭草;

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1. Crystallization and preliminary X-ray crystallographic studies of a Lys49-phospholipase A2 homologue from Bothrops pirajai venom complexed with rosmarinic acid [J] . Juliana I. Dos Santos Norival A. Santos-Filho Andreimar M. Soares Marcos R. M. Fontes Acta Crystallographica Section F . 2010,第6期

机译：结合迷迭香酸的Bothrops pirajai毒液中Lys49-磷脂酶A2同源物的结晶和初步X射线晶体学研究
2. Preliminary X-Ray Crystallographic Studies of a Lys49-Phospholipase A2 Homologue from Bothrops pirajai Venom Complexed with p-Bromophenacyl Bromide and α-Tocopherol Inhibitors [J] . Juliana I. dos Santos Daniela P. Marchi-Salvador Carlos A.H. Fernandes Lucas B. Silveira Andreimar M. Soares Marcos R.M. Fontes Protein and Peptide Letters . 2007,第7期

机译：结合了对溴苯甲酰基溴化物和α-生育酚抑制剂的Bothrops pirajai毒液中Lys49-磷脂酶A2同源物的初步X射线晶体学研究
3. Preliminary X-Ray Crystallographic Studies of a Lys49 Phospholipase A2 Homologue from Bothrops pirajai Venom Complexed with p-Bromo-phenacyl Bromide and α Tocopherol Inhibitors [J] . J.I. dos Santos, D.P. Marchi-Salvador, C.A.H. Fernandes, Protein and peptide letters . 2007,第7期

机译：Both虫与双溴苯甲酰溴和α-生育酚抑制剂复合的Lys49磷脂酶A2同源物的初步X射线晶体学研究
4. Synthesis, Characterization and X-Ray Crystallographic Investigation of Zn(II) beta-Diketo Tetronic Acid Complexes [C] . Stefanou, V., Prousis, European Biological Inorganic Chemistry Conference . 2010

机译：Zn（II）β-Diketo苯乙酸复合物的合成，表征和X射线晶体研究
5. Reactivity of sandwich pi-complexes towards dithiocarboxylic acids: Synthesis and x-ray crystallographic studies of titanium, vanadium, and niobium inorganic/organometallic sulfur-containing complexes [D] . Andras, Maria Theresa. 1990

机译：三明治型pi配合物对二硫代羧酸的反应活性：钛，钒和铌无机/有机金属含硫配合物的合成和x射线晶体学研究
6. Crystallization and preliminary X-ray diffraction analysis of a Lys49-phospholipase A2 complexed with caffeic acid a molecule with inhibitory properties against snake venoms [O] . Patrícia S. Shimabuku, Carlos A. H. Fernandes, Angelo J. Magro, 2011

机译：与咖啡酸复合的Lys49-磷脂酶A2的结晶和初步X射线衍射分析咖啡酸是一种对蛇毒具有抑制作用的分子
7. Crystallization and preliminary X-ray crystallographic studies of a Lys49-phospholipase A2 homologue from Bothrops pirajai venom complexed with rosmarinic acid [O] . dos Santos, Juliana I., Santos-Filho, Norival A., Soares, Andreimar M., 2010

机译：结合迷迭香酸的Bothrops pirajai毒液中Lys49-磷脂酶A2同源物的结晶和初步X射线晶体学研究
8. Crystallographic Studies of Drug--Nucleic Acid Crystalline Complexes and Their Biological Implications [R] . Sobell, H. M., Jain, S. C., Sakore, T. D., 1978

机译：药物 - 核酸结晶复合物的晶体学研究及其生物学意义

Crystallization and preliminary X-ray crystallographic studies of a Lys49-phospholipase A2 homologue from Bothrops pirajai venom complexed with rosmarinic acid

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