Crystallization and preliminary X-ray data collection of the L27PATJ–(L27NL27C)Pals1–L27MALS tripartite complex

机译：L27PATJ–（L27NL27C）Pals1–L27MALS三方复合物的结晶和初步X射线数据收集

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摘要

The L27 (LIN-2/LIN-7) domain is a protein–protein interaction module capable of assembling proteins into biologically important complexes. Pals1 contains two L27 domains: the first, L27N, interacts with PATJ, and the second, L27C, interacts with MALS, forming a tripartite complex that plays a crucial role in the establishment and maintenance of cell polarity. To provide a better understanding of the mechanism of assembly of this tripartite complex, four different L27PATJ–(L27N,L27C)Pals1–L27MALS constructs were cloned, expressed, purified and crystallized. Crystals of tripartite complex 1 of L27PATJ–(L27N,L27C)Pals1–L27MALS diffracted to 2.05 Å resolution. These crystals belonged to either space group P6122 or P6522, with unit-cell parameters a = b = 145.2, c = 202.5 Å. Assuming the presence of four molecules in the asymmetric unit, a Matthews coefficient of 2.69 Å³ Da⁻¹ was calculated, corresponding to a solvent content of 54.25%.

机译：L27（LIN-2 / LIN-7）域是一种蛋白质-蛋白质相互作用模块，能够将蛋白质组装成生物学上重要的复合物。 Pals1包含两个L27域：第一个L27N与PATJ相互作用，第二个L27C与MALS相互作用，形成三方复合物，在建立和维持细胞极性中起关键作用。为了更好地了解这种三方复合物的组装机理，我们克隆，表达，纯化和结晶了四种不同的L27PATJ-（L27N，L27C）Pals1-L27MALS构建体。 L27PATJ–（L27N，L27C）Pals1–L27MALS的三方配合物1的晶体衍射至2.05Å分辨率。这些晶体属于空间群P6122或P6522，其晶胞参数a = b = 145.2，c = 202.5。假设在不对称单元中存在四个分子，计算出的马修斯系数为2.69Åsup<3 Da ^{-1 ，对应的溶剂含量为54.25％。}

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期刊名称 Acta Crystallographica Section F: Structural Biology and Crystallization Communications
作者
Jinxiu Zhang; Xue Yang; Yuequan Shen; Jiafu Long;
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作者单位

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年(卷),期 2011(67),Pt 11
年度 2011
页码 1443–1447
总页数 5
原文格式 PDF
正文语种
中图分类分子生物学;生化遗传学;生化药理学;生物物理学;
关键词
LIN-2/LIN-7 domains Pals1 PATJ MALS;

机译：LIN-2 / LIN-7域;Pals1;PAJ;MALS;

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Crystallization and preliminary X-ray data collection of the L27PATJ–(L27NL27C)Pals1–L27MALS tripartite complex

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