Expression purification crystallization and preliminary X-ray diffraction analysis of carbonyl reductase from Candida parapsilosis ATCC 7330

机译：表达纯化结晶和来自Candida羰基还原酶的初步X射线衍射分析近平滑念珠菌aTCC 7330

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The NAD(P)H-dependent carbonyl reductase from Candida parapsilosis ATCC 7330 catalyses the asymmetric reduction of ethyl 4-phenyl-2-oxobutanoate to ethyl (R)-4-phenyl-2-hydroxybutanoate, a precursor of angiotensin-converting enzyme inhibitors such as Cilazapril and Benazepril. The carbonyl reductase was expressed in Escherichia coli and purified by GST-affinity and size-exclusion chromatography. Crystals were obtained by the hanging-drop vapour-diffusion method and diffracted to 1.86 Å resolution. The asymmetric unit contained two molecules of carbonyl reductase, with a solvent content of 48%. The structure was solved by molecular replacement using cinnamyl alcohol dehydrogenase from Saccharomyces cerevisiae as a search model.

机译：来自副念珠菌ATCC 7330的NAD（P）H依赖性羰基还原酶催化4-苯基-2-氧代丁酸乙酯不对称还原为（R）-4-苯基-2-羟基丁酸乙酯（血管紧张素转换酶抑制剂的前体）如西拉普利和贝那普利。羰基还原酶在大肠杆菌中表达，并通过GST亲和力和尺寸排阻色谱法纯化。通过悬滴蒸气扩散法获得晶体，并衍射至1.86分辨率。不对称单元包含两个分子的羰基还原酶，溶剂含量为48％。通过使用来自酿酒酵母的肉桂醇脱氢酶作为搜索模型，通过分子置换来解决结构。

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期刊名称 Acta Crystallographica Section F: Structural Biology and Crystallization Communications
作者
Nidhi Aggarwal; P. K. Mandal; Namasivayam Gautham; Anju Chadha;
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作者单位

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年(卷),期 2013(69),Pt 3
年度 2013
页码 313–315
总页数 3
原文格式 PDF
正文语种
中图分类分子生物学;生化遗传学;生化药理学;生物物理学;
关键词
carbonyl reductase alcohol dehydrogenase enantioselectivity Candida parapsilosis;

机译：羰基还原酶;醇脱氢酶;对映选择性;副念珠菌;

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专利

1. Expression, purification, crystallization and preliminary X-ray diffraction analysis of carbonyl reductase from Candida parapsilosis ATCC 7330 [J] . Aggarwal Nidhi, Mandal P. K., Gautham Namasivayam, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第3期

机译：副念珠菌ATCC 7330中羰基还原酶的表达，纯化，结晶和初步X射线衍射分析
2. A carbonyl reductase from Candida parapsilosis ATCC 7330: substrate selectivity and enantiospecificity [J] . Sneha Sudhakara, Anju Chadha Organic & biomolecular chemistry . 2017,第19期

机译：副念珠菌ATCC 7330的羰基还原酶：底物选择性和对映体特异性
3. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of AerF from Microcystis aeruginosa, a putative reductase participating in aeruginosin biosynthesis [J] . Ding Ruyi, Xu Cui, Chen Xu, Acta crystallographica. Section F, Structural biology communications . 2015,第4期

机译：铜绿微囊藻AerF的克隆，表达，纯化，结晶和初步X射线衍射分析，铜绿微囊藻是一种参与铜绿菌素生物合成的假定还原酶
4. Identification and Characterization of Novel Anti-Prelog Carbonyl Reductases Involved in Candida parapsilosis Catalyzing One-Pot Stereoinversion of l-Phenyl-l,2-ethanediol [C] . Yao Nie, Yan Xu, Zhen Yan, China-Japan-Korea joint symposium on enzyme engineering . 2010

机译：新型假丝酵母催化的一锅立体转化1-苯基-1,2-乙二醇的抗前序羰基还原酶的鉴定与表征
5. Expression, purification, crystallization and preliminary X-ray analysis of PR-AMP cyclohydrolase, a zinc binding enzyme from Methanobacterium thermoautotrophicum. [D] . Boju, Lorena. 2004

机译：PR-AMP环水解酶的表达，纯化，结晶和初步X射线分析，PR-AMP环水解酶是一种来自嗜热自养甲烷杆菌的锌结合酶。
6. Crystallization and preliminary X-ray diffraction analysis of (R)-carbonyl reductase from Candida parapsilosis [O] . Shanshan Wang, Yao Nie, Xu Yan, 2014

机译：副念珠菌（R）-羰基还原酶的结晶和初步X射线衍射分析
7. Expression, purification, crystallization and preliminary X-ray analysis of conjugated polyketone reductase C2 (CPR-C2) from Candida parapsilosis IFO 0708 [O] . Yamamura, Akihiro, Maruoka, Shintaro, Ohtsuka, Jun, 2009

机译：副念珠菌IFO 0708的共轭聚酮还原酶C2（CPR-C2）的表达，纯化，结晶和初步X射线分析

Expression purification crystallization and preliminary X-ray diffraction analysis of carbonyl reductase from Candida parapsilosis ATCC 7330

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