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A review on SNP and other types of molecular markers and their use in animal genetics

机译:SNP和其他类型的分子标记及其在动物遗传学中的应用综述

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摘要

During the last ten years, the use of molecular markers, revealing polymorphism at the DNA level, has been playing an increasing part in animal genetics studies. Amongst others, the microsatellite DNA marker has been the most widely used, due to its easy use by simple PCR, followed by a denaturing gel electrophoresis for allele size determination, and to the high degree of information provided by its large number of alleles per locus. Despite this, a new marker type, named SNP, for Single Nucleotide Polymorphism, is now on the scene and has gained high popularity, even though it is only a bi-allelic type of marker. In this review, we will discuss the reasons for this apparent step backwards, and the pertinence of the use of SNPs in animal genetics, in comparison with other marker types.
机译:在过去的十年中,分子标记的使用在DNA水平揭示了多态性,在动物遗传学研究中的作用日益增加。其中,微卫星DNA标记已得到最广泛的应用,这是因为它易于通过简单的PCR进行使用,随后通过变性凝胶电泳来确定等位基因大小,并且由于每个位点具有大量等位基因而提供了高度信息。尽管如此,一种新的名为单核苷酸多态性的标记物SNP现已出现并获得了很高的知名度,尽管它只是双等位基因类型的标记物。在这篇综述中,我们将讨论这种明显倒退的原因,以及与其他标记物类型相比在动物遗传学中使用SNP的相关性。

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