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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Comparison of Paenibacillus azotofixans Strains Isolated from Rhizoplane, Rhizosphere, and Non-Root-Associated Soil from Maize Planted in Two Different Brazilian Soils
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Comparison of Paenibacillus azotofixans Strains Isolated from Rhizoplane, Rhizosphere, and Non-Root-Associated Soil from Maize Planted in Two Different Brazilian Soils

机译：从两种不同巴西土壤中种植的玉米的根际，根际和非根系土壤分离的固氮芽孢杆菌菌株的比较

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Paenibacillus azotofixans is a nitrogen-fixing bacterium often found in soil and in the rhizospheres of different grasses. In this study, two Brazilian clay soils were planted with cross-hybrid maize (BR-201) and four stages of plant growth were analyzed to characterize the P. azotofixans populations present in the rhizoplanes, rhizospheres, and non-root-associated soils (herein called nonrhizospheres). A total of 106 strains were isolated and identified as P. azotofixans with an API 50CH kit, by classical biochemical tests, and via the use of specific primers based on the 16S rRNA gene in PCRs. To compare the isolated strains, phenotypic characteristics were determined and three different probes were used in hybridization experiments: two nif probes and one probe comprising a 0.58-kb fragment cloned from the P. azotofixans C3L4 genome. These results were used to construct a dendrogram, in which two main clusters could be observed. One cluster contained exclusively strains from Várzea soil, and the other contained the majority of strains from Cerrado soil. The 60 strains from Várzea soil and the 46 strains from Cerrado soil were further analyzed with REP and BOX primers, respectively. Based on the patterns obtained, it was possible to identify 21 different groups among strains from Várzea soil and 4 different groups among strains from Cerrado soil. These different patterns were tested by multivariate analysis of variance, and differences in the populations of P. azotofixans during the four stages of plant growth were demonstrated. Moreover, strains isolated from the rhizoplanes, rhizospheres, and nonrhizospheres of maize planted in Cerrado and Várzea soils were shown to be statistically different; the diversity of P. azotofixans strains was affected by the soil type.
机译：固氮芽孢杆菌是一种固氮细菌，经常在土壤和不同草的根际中发现。在这项研究中，在两种巴西黏土上种植了杂交玉米（BR-201），并分析了植物的四个生长阶段，以表征在根际平面，根际和非根系土壤中存在的固氮假单胞菌种群（这里称为非根际）。通过经典的生化测试，并使用基于16S rRNA基因的特异性引物在PCR中使用API​​ 50CH试剂盒，共分离出106株菌株并将其鉴定为偶氮假单胞菌。为了比较分离的菌株，确定了表型特征，并在杂交实验中使用了三种不同的探针：两种nif探针和一种包含从偶氮固硫杆菌C3L4基因组克隆的0.58-kb片段的探针。这些结果被用于构建树状图，其中可以观察到两个主要簇。一个簇仅包含来自Várzea土壤的菌株，另一个簇包含来自Cerrado土壤的大多数菌株。用REP和BOX引物分别分析了Várzea土壤的60株和Cerrado土壤的46株。根据获得的模式，有可能在Várzea土壤的菌株中鉴定出21个不同的组，在Cerrado土壤的菌株中鉴定出4个不同的组。通过方差的多变量分析测试了这些不同的模式，并证明了植物生长四个阶段中偶氮固硫假单胞菌种群的差异。而且，从Cerrado和Várzea土壤中种植的玉米的根际，根际和非根际分离出的菌株在统计学上是不同的。土壤类型影响了固氮假单胞菌菌株的多样性。

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