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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Molecular Detection of Campylobacter spp. and Fecal Indicator Bacteria during the Northern Migration of Sandhill Cranes (Grus canadensis) at the Central Platte River
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Molecular Detection of Campylobacter spp. and Fecal Indicator Bacteria during the Northern Migration of Sandhill Cranes (Grus canadensis) at the Central Platte River

机译：弯曲杆菌属的分子检测。普拉特河中部沙丘鹤（Grus canadensis）向北迁移期间的细菌和粪便指示菌

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The risk to human health of the annual sandhill crane (Grus canadensis) migration through Nebraska, which is thought to be a major source of fecal pollution of the central Platte River, is unknown. To better understand potential risks, the presence of Campylobacter species and three fecal indicator bacterial groups (Enterococcus spp., Escherichia coli, and Bacteroidetes) was assayed by PCR from crane excreta and water samples collected during their stopover at the Platte River, Nebraska, in 2010. Genus-specific PCR assays and sequence analyses identified Campylobacter jejuni as the predominant Campylobacter species in sandhill crane excreta. Campylobacter spp. were detected in 48% of crane excreta, 24% of water samples, and 11% of sediment samples. The estimated densities of Enterococcus spp. were highest in excreta samples (mean, 4.6 × 108 cell equivalents [CE]/g), while water samples contained higher levels of Bacteroidetes (mean, 5.1 × 105 CE/100 ml). Enterococcus spp., E. coli, and Campylobacter spp. were significantly increased in river water and sediments during the crane migration period, with Enterococcus sp. densities (∼3.3 × 105 CE/g) 2 to 4 orders of magnitude higher than those of Bacteroidetes (4.9 × 103 CE/g), E. coli (2.2 × 103 CE/g), and Campylobacter spp. (37 CE/g). Sequencing data for the 16S rRNA gene and Campylobacter species-specific PCR assays indicated that C. jejuni was the major Campylobacter species present in water, sediments, and crane excreta. Overall, migration appeared to result in a significant, but temporary, change in water quality in spring, when there may be a C. jejuni health hazard associated with water and crops visited by the migrating birds.
机译：尚不知道每年通过内布拉斯加州的沙丘鹤（加拿大鹤）迁移对人类健康的风险，这被认为是普拉特河中部粪便污染的主要来源。为了更好地了解潜在风险，通过PCR分析了内布拉斯加州普拉特河停泊期间起重机起重机的粪便和收集的水样中的弯曲杆菌属和三个粪便指示菌群（肠球菌，大肠杆菌和拟杆菌）的存在。 2010。属特异性PCR分析和序列分析确定空肠弯曲杆菌为沙丘鹤排泄物中的主要弯曲杆菌。弯曲杆菌属。在48％的起重机粪便，24％的水样品和11％的沉积物样品中检测到了这些污染物。肠球菌的估计密度。排泄物样品中最高（平均4.6×10 8 细胞当量[CE] / g），而水样中的拟杆菌含量较高（平均5.1×10 5 ） CE / 100毫升）。肠球菌属，大肠杆菌和弯曲杆菌属。在起重机迁移期间，河水和沉积物的含量显着增加，肠球菌sp.。大肠杆菌的密度（〜3.3×10 5 CE / g）比拟杆菌（4.9×10 3 CE / g）高2至4个数量级2.2×10 3 CE / g）和弯曲杆菌 spp。 （37 CE / g）。 16S rRNA基因和 Campylobacter 物种特异性PCR分析的测序数据表明， C。空肠是水，沉积物和鹤类排泄物中主要的弯曲杆菌。总体而言，迁移可能会导致春季的水质发生重大但暂时的变化，此时可能会出现 C。与迁徙鸟类探访的水和农作物有关的空肠健康危害。

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