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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Living Side by Side with a Virus: Characterization of Two Novel Plasmids from Thermococcus prieurii, a Host for the Spindle-Shaped Virus TPV1
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Living Side by Side with a Virus: Characterization of Two Novel Plasmids from Thermococcus prieurii, a Host for the Spindle-Shaped Virus TPV1

机译：与病毒并存：纺锤形病毒TPV1宿主热球菌Prieurii的两个新质粒的表征

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摘要

Microbial cells often serve as an evolutionary battlefield for different types of mobile genetic elements, such as viruses and plasmids. Here, we describe the isolation and characterization of two new archaeal plasmids which share the host with the spindle-shaped Thermococcus prieurii virus 1 (TPV1). The two plasmids, pTP1 and pTP2, were isolated from the hyperthermophilic archaeon Thermococcus prieurii (phylum Euryarchaeota), a resident of a deep-sea hydrothermal vent located at the East Pacific Rise at 2,700-m depth (7°25′24 S, 107°47′66 W). pTP1 (3.1 kb) and pTP2 (2.0 kb) are among the smallest known plasmids of hyperthermophilic archaea, and both are predicted to replicate via the rolling-circle mechanism. The two plasmids and the virus TPV1 do not have a single gene in common and stably propagate in infected cells without any apparent antagonistic effect on each other. The compatibility of the three genetic elements and the high copy number of pTP1 and pTP2 plasmids (50 copies/cell) might be useful for developing new genetic tools for studying hyperthermophilic euryarchaea and their viruses.
机译：微生物细胞通常充当不同类型的移动遗传元件（例如病毒和质粒）的进化战场。在这里，我们描述了两个新的古细菌质粒的分离和表征，它们与纺锤形的普氏热球菌病毒1（TPV1）共享宿主。两种质粒pTP1和pTP2是从超嗜热古细菌Thermococcus prieurii（phylum Euryarchaeota）分离的，该物种位于东太平洋上升深海热液喷口处，位于2,700米深度（7°25′24 S，107） °47′66 W）。 pTP1（3.1 kb）和pTP2（2.0 kb）是嗜热古生菌中最小的已知质粒，预计它们都将通过滚环机制复制。这两个质粒和病毒TPV1没有共同的单一基因，并且在感染的细胞中稳定繁殖，彼此之间没有明显的拮抗作用。这三个遗传因素的兼容性以及pTP1和pTP2质粒的高拷贝数（50个拷贝/细胞）可能有助于开发新的遗传工具，用于研究嗜热性古菌及其病毒。

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