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Comparative Phenotype and Genome Analysis of Cellvibrio sp. PR1, a Xylanolytic and Agarolytic Bacterium from the Pearl River

机译:Cellvibrio sp。的比较表型和基因组分析。 PR1,来自珠江三角洲的木糖分解和糖化细菌

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摘要

Cellvibrio sp. PR1 is a xylanolytic and agarolytic bacterium isolated from the Pearl River. Strain PR1 is closely related to Cellvibrio fibrivorans and C. ostraviensis (identity > 98%). The xylanase and agarase contents of strain PR1 reach up to 15.4 and 25.9 U/mL, respectively. The major cellular fatty acids consisted of C16:0 (36.7%), C18:0 (8.8%), C20:0 (6.8%), C15:0 iso 2-OH or/and C16:1ω7c (17.4%), and C18:1ω7c or/and C18:1ω6c (6.7%). A total of 251 CAZyme modules (63 CBMs, 20 CEs, 128 GHs, 38 GTs, and 2 PLs) were identified from 3,730 predicted proteins. Genomic analysis suggested that strain PR1 has a complete xylan-hydrolyzing (5 β-xylanases, 16 β-xylosidases, 17 α-arabinofuranosidases, 9 acetyl xylan esterases, 4 α-glucuronidases, and 2 ferulic acid esterases) and agar-hydrolyzing enzyme system (2 β-agarases and 2 α-neoagarooligosaccharide hydrolases). In addition, the main metabolic pathways of xylose, arabinose, and galactose are established in the genome-wide analysis. This study shows that strain PR1 contains a large number of glycoside hydrolases.
机译:细胞弧菌PR1是一种从珠江分离的木聚糖分解和琼脂分解细菌。 PR1菌株与纤维弧菌和C. ostraviensis密切相关(同一性> 98%)。 PR1菌株的木聚糖酶和琼脂酶含量分别达到15.4和25.9 U / mL。主要的细胞脂肪酸由C16:0(36.7%),C18:0(8.8%),C20:0(6.8%),C15:0 iso 2-OH或/和C16:1ω7c(17.4%)和C18:1ω7c或/和C18:1ω6c(6.7%)。从3,730个预测的蛋白质中鉴定出总共251个CAZyme模块(63个CBM,20个CE,128个GH,38个GT和2个PL)。基因组分析表明,菌株PR1具有完整的木聚糖水解酶(5种木聚糖酶,16种木糖苷酶,17种α-阿拉伯呋喃糖苷酶,9种乙酰木聚糖酯酶,4种α-葡糖醛酸糖苷酶和2种阿魏酸酯酶)和琼脂水解酶系统(2个β-琼脂糖和2个α-新葡糖寡糖水解酶)。另外,在全基因组分析中建立了木糖,阿拉伯糖和半乳糖的主要代谢途径。这项研究表明,菌株PR1含有大量的糖苷水解酶。

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