A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes

机译：用于模式生物基因组的CRISPR / Cas9系统的指导RNA序列设计平台

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摘要

Cas9/CRISPR has been reported to efficiently induce targeted gene disruption and homologous recombination in both prokaryotic and eukaryotic cells. Thus, we developed a Guide RNA Sequence Design Platform for the Cas9/CRISPR silencing system for model organisms. The platform is easy to use for gRNA design with input query sequences. It finds potential targets by PAM and ranks them according to factors including uniqueness, SNP, RNA secondary structure, and AT content. The platform allows users to upload and share their experimental results. In addition, most guide RNA sequences from published papers have been put into our database.

机译：据报道，Cas9 / CRISPR在原核和真核细胞中均能有效诱导靶向基因破坏和同源重组。因此，我们为模型生物的Cas9 / CRISPR沉默系统开发了一个指导RNA序列设计平台。该平台易于使用输入查询序列进行gRNA设计。它通过PAM查找潜在目标，并根据包括唯一性，SNP，RNA二级结构和AT含量在内的因素对它们进行排名。该平台允许用户上传并分享他们的实验结果。此外，大部分来自已发表论文的指南RNA序列都已放入我们的数据库中。

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期刊名称 BioMed Research International
作者
Ming Ma; Adam Y. Ye; Weiguo Zheng; Lei Kong;
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作者单位

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年(卷),期 2006(2013),
年度 2006
页码 270805
总页数 4
原文格式 PDF
正文语种
中图分类生物学;
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专利

1. A Guide RNA Sequence Design Platform for the CRISPM/Cas9 System for Model Organism Genomes [J] . Ming Ma, Adam Y. Ye, Weiguo Zheng, BioMed research international . 2013,第Pta24期

机译：用于模型生物基因组的CRISPM / Cas9系统的指导性RNA序列设计平台
2. Enhanced guide-RNA design and targeting analysis for precise CRISPR genome editing of single and consortia of industrially relevant and non-model organisms [J] . Brian JMendoza, Cong TTrinh Bioinformatics . 2018,第1期

机译：增强的指导RNA设计和靶向分析，用于单一和非模型生物的单一和联盟的精确克里普尔基因组编辑
3. CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences [J] . Lin Yanni, Cradick Thomas J., Brown Matthew T., Nucleic Acids Research . 2014,第11期

机译：CRISPR / CAS9系统在靶DNA与指导RNA序列之间的插入或缺失具有脱靶活动
4. DASE: Condition-specific differential alternative splicing variants estimation method without reference genome sequence, and its application to non-model organisms [C] . Kouki Yonezawa, Tsukasa Mori, Shuichi Shigeno, IEEE International Conference on Bioinformatics and Biomedicine . 2016

机译：DASE：无参考基因组序列的条件特异性差异替代剪接变体估计方法，其在非模式生物中的应用
5. Chromothripsis as an On-Target Consequence of CRISPR–Cas9 Genome Editing [D] . Leibowitz, Mitchell L. 2021

机译：Chromothripsis作为CRISPR-CAS9基因组编辑的目标后果
6. CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences [O] . Yanni Lin, Thomas J. Cradick, Matthew T. Brown, -1

机译：CRISPR / Cas9系统具有脱靶活性在靶DNA和指导RNA序列之间有插入或缺失
7. CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences [O] . Y. Lin, T. J. Cradick, M. T. Brown, 2014

机译：CRISPR / CAS9系统在靶DNA与指导RNA序列之间的插入或缺失具有脱靶活动

A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes

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