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Direct and Rapid Description of the Individual Ionic Currents of Squid Axon Membrane by Ramp Potential Control

机译:通过斜坡电位控制直接快速描述鱿鱼轴突膜的各个离子电流

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摘要

Computations based upon the Hodgkin-Huxley equations and experimental data from squid axons show that ramp functions can be used as commands to a voltage clamp system to selectively observe either the fast (sodium) or slow (potassium) process in axon membranes without chemical separation techniques or computer assistance. Each process is characterized directly (on line) and rapidly (real time) by generating a current-potential curve on an oscilloscope for fast or slow rates of change of membrane potential (ramps). The speed and directness of this method of characterizing each of the essential axonal events permit quantitative measurement of the kinetics of rapid effects on these processes due to various pharmacological agents such as tetrodotoxin and tetraethylammonium ion or other experimental changes in the membrane environment.
机译:根据Hodgkin-Huxley方程和鱿鱼轴突的实验数据进行的计算表明,斜坡函数可以用作电压钳系统的命令,从而无需化学分离技术即可选择性观察轴突膜中的快速(钠)或慢速(钾)过程或计算机协助。通过在示波器上生成电流电位曲线以快速或缓慢地改变膜电位(斜坡)的速率,可以直接(在线)和快速(实时)表征每个过程。这种表征每个基本轴突事件的方法的速度和直接性,可以定量测量由于诸如河豚毒素和四乙铵离子等多种药理作用或膜环境中的其他实验变化而对这些过程产生的快速影响的动力学。

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