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首页> 美国卫生研究院文献>BMC Plant Biology >Depletion of the heaviest stable N isotope is associated with NH4+/NH3 toxicity in NH4+-fed plants
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Depletion of the heaviest stable N isotope is associated with NH4+/NH3 toxicity in NH4+-fed plants

机译：饲喂NH4 +的植物中最重的稳定N同位素的消耗与NH4 + / NH3毒性有关

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BackgroundIn plants, nitrate (NO3-) nutrition gives rise to a natural N isotopic signature (δ15N), which correlates with the δ15N of the N source. However, little is known about the relationship between the δ15N of the N source and the 14N/15N fractionation in plants under ammonium (NH4+) nutrition. When NH4+ is the major N source, the two forms, NH4+ and NH3, are present in the nutrient solution. There is a 1.025 thermodynamic isotope effect between NH3 (g) and NH4+ (aq) which drives to a different δ15N. Nine plant species with different NH4+-sensitivities were cultured hydroponically with NO3- or NH4+ as the sole N sources, and plant growth and δ15N were determined. Short-term NH4+/NH3 uptake experiments at pH 6.0 and 9.0 (which favours NH3 form) were carried out in order to support and substantiate our hypothesis. N source fractionation throughout the whole plant was interpreted on the basis of the relative transport of NH4+ and NH3.
机译：背景在植物中，硝酸盐（NO3 -）营养产生天然的N同位素特征（δ 15 N），与δ 15 相关N源中的N个。然而，对于铵态下植物中N源的δ 15 N与 14 N / 15 N分馏之间的关系知之甚少。 （NH4 + ）营养。当NH4 + 是主要的氮源时，营养液中会同时存在NH4 + 和NH3这两种形式。 NH3（g）和NH4 + （aq）之间存在1.025的热力学同位素效应，可驱动不同的δ 15 N。以NO3 -或NH4 + 作为唯一的氮源，对9种具有不同NH4 + 敏感性的植物进行水培。确定了 15 N。为了支持和证实我们的假设，在pH 6.0和9.0（有利于NH3形式）下进行了短期NH4 + / sup / NH3吸收实验。根据NH4 + 和NH3的相对迁移来解释整个植物中的N源分馏。

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