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首页> 美国卫生研究院文献>British Journal of Experimental Pathology >The isolation and characterization of a bile ductule cell population from normal and bile-duct ligated rat livers.
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The isolation and characterization of a bile ductule cell population from normal and bile-duct ligated rat livers.

机译：从正常和胆管结扎的大鼠肝脏中分离和鉴定胆管细胞群。

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摘要

The separation of bile ductule cells from Kupffer and sinusoidal endothelial cells in a suspension of non-parenchymal cells has been attempted. Bile duct ligation was performed so that a four-fold increase in the total number of non-parenchymal cells isolated from rat liver was attained and the proportions of both Kupffer and bile ductule cells were elevated. Rate zonal centrifugation, through a Ficoll gradient, partially separated the cells into two populations: (1) small cells (4-6 micrometer diameter) probably originating from the sinusoidal endothelium and (2) larger bile ductule and Kupffer cells (8-12 micrometer diameter). A more successful separation was achieved by isopycnic centrifugation through a linear metrizamide gradient. Bile duct ligation (14 days) altered the distribution of cells on the gradient and the peak containing bile ductule and Kupffer cells partially subdivided into the separate cell types. Antiserum raised against the bile ductule fraction was shown to be compatible with that raised against common bile duct tissue. gamma-glutamyl transpeptidase and leucine aminopeptidase activity were preferentially located in the rate zonal fraction containing bile ductule cells. Their specific activity increased after bile duct ligation as did that of beta-glucuronidase, which was raised in all cells.
机译：已尝试在非实质细胞悬浮液中从库普弗和窦性内皮细胞中分离胆管细胞。进行胆管结扎，以使从大鼠肝脏分离的非实质细胞总数增加四倍，并且库普弗和胆管细胞的比例均升高。通过Ficoll梯度速率区域离心将细胞部分分成两个群体：（1）小细胞（直径4-6微米）可能源自正弦内皮细胞；（2）大胆管和库普弗细胞（8-12微米）直径）。通过线性甲氨amide呤梯度等速离心，可以实现更成功的分离。胆管结扎（14天）改变了梯度细胞的分布，含有胆管和库普弗细胞的峰部分细分为不同的细胞类型。对抗胆管部分产生的抗血清被证明与针对胆总管组织产生的抗血清相容。 γ-谷氨酰转肽酶和亮氨酸氨肽酶的活性优先位于含有胆管细胞的带状区域。它们的比活性在胆管结扎后增加，如β-葡萄糖醛酸苷酶在所有细胞中均升高。

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