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首页> 美国卫生研究院文献>Canadian Journal of Comparative Medicine >Improvements in the modified direct complement fixation test and its application in the detection of bluetongue antibodies in cattle and sheep sera.
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Improvements in the modified direct complement fixation test and its application in the detection of bluetongue antibodies in cattle and sheep sera.

机译：改进的直接补体固定试验的改进及其在牛和绵羊血清中蓝舌抗体检测中的应用。

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In this study, improvements were made in the technique and the preparation of the antigen. It was possible to perform three extractions and elutions resulting in a soluble reactive preparation from each batch of infected mouse brain. This led to an appreciable increase in the yield of highly reactive antigen. The presence of bluetongue antibodies was not detected in 13,210 sheep sera. Of the 13,486 bovine sera tested, only three questionable reactions were obtained. It was possible to determine that two of these animals were imported. Various isolation methods, including transmission trials to susceptible sheep followed by serological tests on the sheep sera, failed to confirm the infection in the three reactors.
机译：在这项研究中，对抗原的技术和制备进行了改进。可以进行三次萃取和洗脱，从每批受感染的小鼠脑中产生可溶的反应性制剂。这导致高反应性抗原的产量明显增加。在13,210只绵羊血清中未检测到蓝舌抗体的存在。在测试的13486种牛血清中，仅获得了三个可疑的反应。可以确定其中有两只动物是进口的。各种隔离方法，包括向易感羊的传播试验，然后对绵羊血清进行血清学检测，均未能证实这三个反应堆中的感染。

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