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首页> 美国卫生研究院文献>Canadian Journal of Comparative Medicine >Evaluation of adipose-derived stromal vascular fraction from the lateral tailhead inguinal region and mesentery of horses
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Evaluation of adipose-derived stromal vascular fraction from the lateral tailhead inguinal region and mesentery of horses

机译：评估马尾巴腹股沟区和肠系膜脂肪来源的基质血管分数

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摘要

Use of mesenchymal stem cells (MSCs) found in the stromal vascular fraction (SVF) of equine adipose tissue has promising applications for regenerative therapies. The most commonly used source of equine adipose tissue is the subcutaneous tailhead. The objective of this study was to compare 3 adipose depot sites in horses and determine the viability and cellular yield, capillary density, gene expression for selected markers, and colony-forming unit fibroblasts (CFU-Fs) in adipose tissue taken from these sites. Adipose tissue was excised from the area lateral to the tailhead, the inguinal region, and the small colon mesentery of 6 horses. Lipoaspirate was also collected from the area lateral to the tailhead. Stromal vascular fraction (SVF) was prepared in duplicate from the 3 different adipose tissue depots. The total nucleated and dead cell counts was determined manually using a hemocytometer and percent viability was calculated. Mass and volume of adipose were determined in order to calculate density and factor-VIII immunohistochemical staining was used to determine vascular density in the excisional adipose tissue samples from each horse. Quantitative polymerase chain reaction (qPCR) was used to quantify gene expression for selected cellular markers from each site. There were significant differences in viability, yield of nucleated cells/gram of adipose tissue, vascular density, gene expression, and CFU-Fs among adipose depots. Adipose from the mesentery yielded the highest number of nucleated cells/gram of tissue and the highest vascular density and percentage of CFU-Fs. In the horse, both the anatomical site of collection and the method of tissue collection significantly impact the yield and composition of cells in the SVF. Further study is needed to assess whether one adipose source is superior for harvesting mesenchymal stem cells (MSCs) and whether the differences among sources are clinically relevant for in-vivo treatment of musculoskeletal injuries in horses.
机译：在马脂肪组织的间质血管部分（SVF）中发现的间充质干细胞（MSCs）的使用在再生疗法中具有广阔的应用前景。马脂肪组织最常用的来源是皮下尾巴。这项研究的目的是比较马中3个脂肪库的位点，并确定从这些位点获取的脂肪组织中的活力和细胞产量，毛细血管密度，选定标记的基因表达以及集落形成单位成纤维细胞（CFU-Fs）。从6头马的尾巴外侧，腹股沟区域和小结肠肠系膜切下脂肪组织。还从尾部外侧区域收集脂肪油酸酯。从3个不同的脂肪组织贮库中一式两份制备基质血管级分（SVF）。使用血球计数器手动确定总有核和死细胞计数，并计算存活率。确定脂肪的质量和体积以计算密度，并使用VIII因子免疫组化染色确定每匹马切除的脂肪组织样品中的血管密度。定量聚合酶链反应（qPCR）用于量化来自每个位点的选定细胞标记物的基因表达。脂肪库之间的活力，成核细胞的产量/每克脂肪组织，血管密度，基因表达和CFU-Fs存在显着差异。肠系膜中的脂肪产生最多的有核细胞/每克组织，并且产生最高的血管密度和CFU-Fs百分比。在马中，收集的解剖部位和组织收集方法都显着影响SVF中细胞的产量和组成。需要进行进一步的研究，以评估一种脂肪来源是否比收获间充质干细胞（MSC）更优越，以及来源之间的差异是否与马肌肉骨骼损伤的体内治疗在临床上相关。

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