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首页> 美国卫生研究院文献>Cell Regulation >Expression of an activated rasD gene changes cell fate decisions during Dictyostelium development.
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Expression of an activated rasD gene changes cell fate decisions during Dictyostelium development.

机译：激活的rasD基因的表达会在Dictyostelium发育过程中改变细胞命运的决定。

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摘要

It has been previously demonstrated that the expression of an activated rasD gene in wild-type Dictyostelium cells results in formation of aggregates with multitips, instead of the normal single tips, and a block in further development. In an attempt to better understand the role of activated RasD development, we examined cell-type-specific gene expression in a strain stably expressing high levels of RasD[G12T]. We found that the expression of prestalk cell-specific genes ecmA and tagB was markedly enhanced, whereas the expression of the prespore cell-specific gene cotC was reduced to very low levels. When the fate of cells in the multitipped aggregate was monitored with an ecmA/lacZ fusion, it appeared that most of the cells eventually adopted prestalk gene expression characteristics. When mixtures of the [G12T]rasD cells and Ax3 cells were induced to differentiate, chimeric pseudoplasmodia were not formed. Thus, although the [G12T]rasD transformant had a marked propensity to form prestalk cells, it could not supply the prestalk cell population when mixed with wild-type cells. Both stalk and spore cell formation occurred in low cell density monolayers of the [G12T]rasD strain, suggesting that at least part of the inhibition of stalk and spore formation during multicellular development involved inhibitory cell interactions within the cell mass. Models for the possible role of rasD in development are discussed.
机译：先前已经证明，活化的rasD基因在野生型盘基网柄细胞中的表达导致形成具有多尖端而不是正常单尖端的聚集体，并阻止进一步发展。为了更好地理解激活的RasD发育的作用，我们研究了稳定表达高水平RasD [G12T]的菌株中细胞类型特异性基因的表达。我们发现，茎前细胞特异性基因ecmA和tagB的表达明显增强，而芽前细胞特异性基因cotC的表达降低到非常低的水平。当用ecmA / lacZ融合物监测多峰聚集体中细胞的命运时，似乎大多数细胞最终都采用了茎秆前基因表达特征。当诱导[G12T] rasD细胞和Ax3细胞的混合物分化时，不会形成嵌合假质粒。因此，尽管[G12T] rasD转化体具有形成前茎细胞的明显倾向，但是当与野生型细胞混合时，它不能提供前茎细胞群体。茎和孢子细胞的形成均发生在[G12T] rasD菌株的低细胞密度单层中，这表明在多细胞发育过程中至少部分抑制茎和孢子的形成涉及细胞团内的抑制性细胞相互作用。讨论了rasD在开发中可能扮演的角色的模型。

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