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首页> 美国卫生研究院文献>Cell Regulation >Intercellular Organelle Traffic through Cytoplasmic Bridges in Early Spermatids of the Rat: Mechanisms of Haploid Gene Product Sharing
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Intercellular Organelle Traffic through Cytoplasmic Bridges in Early Spermatids of the Rat: Mechanisms of Haploid Gene Product Sharing

机译：早期通过细胞质桥的细胞间细胞器运输。 大鼠的精子：单倍体基因产物的机制 分享分享

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Stable cytoplasmic bridges (or ring canals) connecting the clone of spermatids are assumed to facilitate the sharing of haploid gene products and synchronous development of the cells. We have visualized these cytoplasmic bridges under phase-contrast optics and recorded the sharing of cytoplasmic material between the spermatids by a digital time-lapse imaging system ex vivo. A multitude of small (ca. 0.5 μm) granules were seen to move continuously over the bridges, but only 28% of those entering the bridge were actually transported into other cell. The average speed of the granules decreased significantly during the passage. Immunocytochemistry revealed that some of the shared granules contained haploid cell-specific gene product TRA54. We also demonstrate the novel function for the Golgi complex in acrosome system formation by showing that TRA54 is processed in Golgi complex and is transported into acrosome system of neighboring spermatid. In addition, we propose an intercellular transport function for the male germ cell-specific organelle chromatoid body. This mRNA containing organelle, ca. 1.8 μm in diameter, was demonstrated to go over the cytoplasmic bridge from one spermatid to another. Microtubule inhibitors prevented all organelle movements through the bridges and caused a disintegration of the chromatoid body. This is the first direct demonstration of an organelle traffic through cytoplasmic bridges in mammalian spermatogenesis. Golgi-derived haploid gene products are shared between spermatids, and an active involvement of the chromatoid body in intercellular material transport between round spermatids is proposed.
机译：假定连接精子克隆的稳定细胞质桥（或环管）可促进单倍体基因产物的共享和细胞的同步发育。我们已经在相衬光学下可视化了这些细胞质桥，并通过离体的数字延时成像系统记录了精子之间的细胞质物质共享。观察到许多小的（约0.5μm）颗粒在桥上连续移动，但是进入桥的颗粒中只有28％实际上被转运到其他细胞中。在通过过程中，颗粒的平均速度显着降低。免疫细胞化学显示，某些共有的颗粒含有单倍体细胞特异性基因产物TRA54。我们还通过显示TRA54在高尔基体中加工并被转运到邻近精子的顶体系统中，证明了高尔基体在顶体系统形成中的新功能。此外，我们提出了男性生殖细胞特异性细胞器染色体体的细胞间转运功能。含有mRNA的细胞器。直径1.8μm被证明可以从一个精子穿过细胞质桥到达另一个精子。微管抑制剂可防止所有细胞器运动 通过桥并造成了染色体体的崩解。这个 是细胞质通过细胞质运输的第一个直接证明 哺乳动物精子发生的桥梁。高尔基体来源的单倍体基因产物是 精子之间共享，并活跃于 提出了圆形精子之间的细胞间物质运输。

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