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首页> 美国卫生研究院文献>Clinical and Diagnostic Laboratory Immunology >Investigation of cross-reactions against Trichinella spiralis antigens by enzyme-linked immunosorbent assay and enzyme-linked immunoelectrotransfer blot assay in patients with various diseases.
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Investigation of cross-reactions against Trichinella spiralis antigens by enzyme-linked immunosorbent assay and enzyme-linked immunoelectrotransfer blot assay in patients with various diseases.

机译：通过酶联免疫吸附法和酶联免疫电转移印迹法研究各种疾病患者与旋毛虫旋毛虫抗原的交叉反应。

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Data regarding cross-reactions against Trichinella spiralis in humans are scarce and controversial. For this reason, we tested serum samples from patients with typhoid fever, brucellosis, toxoplasmosis, amoebiasis, cysticercosis, trichocephaliasis, ascariasis, and onchocerciasis against an antigenic extract of T. spiralis infective larvae in an enzyme-linked immunosorbent assay (ELISA) and an enzyme-linked immunoelectrotransfer blot (EITB) assay. All except one serum sample from the group of patients with onchocerciasis were negative in the ELISA; in the EITB assay, only faint bands were observed with the samples from patients with onchocerciasis and ascariasis and negative results were obtained with the samples from patients with other diseases. In conclusion, cross-reactions were found only in the groups of patients with other nematode infections and were of very low magnitude, most of them virtually negative.
机译：关于人类对旋毛虫的交叉反应的数据稀少且有争议。因此，我们通过酶联免疫吸附试验（ELISA）和螺旋藻检测了针对伤寒螺旋体感染性幼虫抗原提取物的伤寒，布鲁氏菌病，弓形虫病，阿米巴虫病，囊虫病，毛癣，as虫病和盘尾丝虫病患者的血清样品。酶联免疫电转移印迹（EITB）分析。 ELISA阴性，除了一种来自盘尾丝虫病患者组的血清样品外，其余均为阴性。在EITB分析中，盘尾丝虫病和as虫病患者的样品仅观察到微弱的条带，而其他疾病患者的样品仅获得阴性结果。总之，交叉反应仅在其他线虫感染的患者中发现，并且反应程度很低，大多数实际上是阴性的。

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